{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:04:14Z","timestamp":1793383454169,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,3,12]],"date-time":"2018-03-12T00:00:00Z","timestamp":1520812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100014943","name":"Beijing Advanced Innovation Center for Food Nutrition and Human Health","doi-asserted-by":"crossref","award":["20181036"],"award-info":[{"award-number":["20181036"]}],"id":[{"id":"10.13039\/501100014943","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In order to circumvent some challenges of the classical approach, the <jats:italic>in silico<\/jats:italic> method has been applied to the discovery of angiotensin\u2010converting enzyme (ACE) inhibitory peptides from food proteins. In this study, some convenient and efficient <jats:italic>in silico<\/jats:italic> tools were utilized to identify novel ACE inhibitory peptides from <jats:styled-content style=\"fixed-case\"><jats:italic>Salmo salar<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Collagen from <jats:styled-content style=\"fixed-case\"><jats:italic>Salmo salar<\/jats:italic><\/jats:styled-content> was digested <jats:italic>in silico<\/jats:italic> into hundreds of peptides. Results revealed that tetrapeptides PGAR and IGPR showed potent ACE inhibitory activity, with IC<jats:sub>50<\/jats:sub> values of 0.598\u2009\u00b1\u20090.12 and 0.43\u2009\u00b1\u20090.09\u2009mmol L<jats:sup>\u22121<\/jats:sup>, respectively. The molecular docking result showed that PGAR and IGPR interact with ACE mostly via hydrogen bonds and attractive charge. Peptide IGPR interacts with Zn<jats:sup>+<\/jats:sup> at the ACE active site, showing high inhibitory activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Interaction with Zn<jats:sup>+<\/jats:sup> in ACE may lead to higher inhibitory activity of peptides, and Pi interactions may promote the effect of peptides on ACE. The <jats:italic>in silico<\/jats:italic> method can be an effective method to predict potent ACE inhibitory peptides from food proteins. \u00a9 2018 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.8908","type":"journal-article","created":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T05:52:14Z","timestamp":1516859534000},"page":"3907-3914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Identification and molecular docking study of novel angiotensin\u2010converting enzyme inhibitory peptides from <scp><i>Salmo salar<\/i><\/scp> using <i>in silico<\/i> methods"],"prefix":"10.1002","volume":"98","author":[{"given":"Zhipeng","family":"Yu","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Bohai University  Jinzhou PR China"},{"name":"Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Technology and Business University (BTBU)  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Bohai University  Jinzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Bohai University  Jinzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Bohai University  Jinzhou PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingbo","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Nutrition and Functional Food Jilin University  Changchun PR 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