{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:59:49Z","timestamp":1784030389652,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T00:00:00Z","timestamp":1526515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"China-Argentina Food Science Technology Center of Chinese Ministry of Science","award":["KY201401005"],"award-info":[{"award-number":["KY201401005"]}]},{"name":"Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences","award":["CAAS-ASTIP-201X-IAPPST"],"award-info":[{"award-number":["CAAS-ASTIP-201X-IAPPST"]}]},{"name":"National Key Research and Development Plan","award":["2016YFD0400205"],"award-info":[{"award-number":["2016YFD0400205"]}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>INTRODUCTION<\/jats:title><jats:p>Peanut stems and leaves (PSL) have traditionally been used as both a special food and a herbal medicine in Asia. The sedative\u2013hypnotic and anxiolytic effects of PSL have been recorded in classical traditional Chinese literature, and more recently by many other researchers. In a previous study, four sleep\u2010related ingredients (linalool, 5\u2010hydroxy\u20104\u2032,7\u2010dimethoxyflavanone, 2\u2032\u2010<jats:italic>O<\/jats:italic>\u2010methylisoliquiritigenin and ferulic acid), among which 5\u2010hydroxy\u20104\u2032,7\u2010dimethoxyflavanone and 2\u2032\u2010<jats:italic>O<\/jats:italic>\u2010methylisoliquiritigenin were newly found in <jats:italic>Arachis<\/jats:italic> species, were screened by ultrahigh\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight mass spectrometry (UHPLC\/QTOF\u2010MS). In the current study, quantitative examination of the above four ingredients was conducted. Serious fundamental functional studies were done in mice, including locomotor activity, direct sleep tests, pentobarbital\u2010induced sleeping time tests, subthreshold dose of pentobarbital tests and barbital sodium sleep incubation period tests, to determine the material base for the sedative\u2013hypnotic and anxiolytic effects of aqueous extracts of PSL. Furthermore, neurotransmitter levels in three brain regions (cerebrum, cerebellum and brain stem) were determined using UHPLC coupled with triple\u2010quadrupole mass spectrometry (UHPLC\/QQQ\u2010MS) in order to elucidate the exact mechanism of action.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Aqueous extract of PSL at a dose of 500 mg kg<jats:sup>\u22121<\/jats:sup> (based on previous experience), along with different concentrations of the above four functional ingredients (189.86 \u00b5g kg<jats:sup>\u22121<\/jats:sup> linalool, 114.75 mg kg<jats:sup>\u22121<\/jats:sup> 5\u2010hydroxy\u20104\u2032,7\u2010dimethoxyflavanone, 32.4mg kg<jats:sup>\u22121<\/jats:sup> 2\u2032\u2010<jats:italic>O<\/jats:italic>\u2010methylisoliquiritigenin and 44.44 mg kg<jats:sup>\u22121<\/jats:sup> ferulic acid), had a sedative\u2212hypnotic effect by affecting neurotransmitter levels in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The data demonstrate that these four ingredients are the key functional factors for the sedative\u2013hypnotic and anxiolytic effects of PSL aqueous extracts and that these effects occur via changes in neurotransmitter levels and pathways. \u00a9 2018 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.9020","type":"journal-article","created":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T03:59:33Z","timestamp":1521863973000},"page":"4885-4894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Sedative\u2013hypnotic and anxiolytic effects and the mechanism of action of aqueous extracts of peanut stems and leaves in mice"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8843-9506","authenticated-orcid":false,"given":"Lei","family":"Deng","sequence":"first","affiliation":[{"name":"Chinese Academy of Agricultural Sciences\/Key Laboratory of Agro\u2010Products 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