{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T11:07:49Z","timestamp":1778065669234,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T00:00:00Z","timestamp":1535673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2019,1,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Appropriate pretreatment strategies that fractionate sugarcane bagasse (SB) are essential for the successful use of this feedstock in ethanol production. In this paper, we investigate a purely enzymatic process to achieve increased production of reducing sugars (RS) from SB in the presence of MgO nanoparticles (MgN) subjected to a three\u2010step sequential enzyme treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULT<\/jats:title><jats:p>Pretreatment of SB with protease activated by magnesium oxide nanoparticles (MgN\u2010pro) at 95 \u00b0C showed an increase in amino acid production by 6.18\u2010fold compared to the untreated enzyme set at the same temperature. High\u2010performance liquid chromatography (HPLC) studies showed an 18\u2010fold removal of lignin from the samples subjected to protease (+ MgN) treatment compared to untreated samples. When the MgN\u2010pro pretreated samples were subjected to pretreatment with xylanase activated by magnesium oxide nanoparticles (MgN\u2010xyl), more than 30\u2010fold increased RS was produced at 8 \u00b0C compared to cellulase (cel) pretreated samples. Xylanase pretreated SB samples produced 1.82\u2010 and 1.91\u2010fold increased reducing sugar and glucose respectively at 8 \u00b0C in the presence of MgN compared to untreated samples at the same temperature.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The results presented here show the efficiency of the proposed method for improving the enzymatic digestibility of SB and explain the pretreatment action mechanism. These findings have potential implications in bio\u2010ethanol, bio\u2010fuel, and agro industries. \u00a9 2018 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.9245","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T02:38:54Z","timestamp":1531363134000},"page":"767-780","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Nanoparticle\u2010induced enzyme pretreatment method for increased glucose production from lignocellulosic biomass under cold conditions"],"prefix":"10.1002","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3609-3259","authenticated-orcid":false,"given":"Nalok","family":"Dutta","sequence":"first","affiliation":[{"name":"Department of Virology, National Institute of Cholera and Enteric Diseases, Kolkata  West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6525-6179","authenticated-orcid":false,"given":"Malay Kumar","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Virology, National Institute of Cholera and Enteric Diseases, Kolkata  West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2007.01.002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-010-8958-4"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(04)70184-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2010.05.002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2012.10.030"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2004.07.001"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1537-7"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2225\/vol13-issue3-fulltext-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137016"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2004.06.025"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8524(01)00103-1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms9091621"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie801542g"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8524(01)00212-7"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260250113"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(03)00123-8"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf035456p"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1385\/ABAB:98-100:1-9:699"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2006.09.062"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00767294"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2008.09.008"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2006.07.015"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/la047994h"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/la00027a034"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2017.08.008"},{"key":"e_1_2_6_27_1","unstructured":"PokoraARandJohnsonMA inventors The Mead Corporation assignee Protease catalyzed treatments of lignocellulose materials. 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