{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T23:44:20Z","timestamp":1773359060693,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T00:00:00Z","timestamp":1557360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Citral is an active component of many plant extracts, and it is a safe additive used in food and cosmetics. A previous study showed that citral has a good antibacterial effect against methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) <jats:italic>in vitro<\/jats:italic>, but its <jats:italic>in vivo<\/jats:italic> anti\u2010infective activity has not been studied. Anti\u2010MRSA activity and the preliminary mechanism of citral against MRSA were investigated in MRSA\u2010infected KM mice. The ED<jats:sub>50<\/jats:sub> was calculated using Karber's method. Groups were selected for inflammatory and oxidative stress level tests, and lung and liver tissues were counterstained with HE for detection of pathological changes. Cytokines and oxidative factors were evaluated using the ELISA method (one\u2010way ANOVA computed using SPSS 19.0.).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>With the increase in the concentration of citral, the survival rate of MRSA\u2010infected mice increased accordingly. The ED<jats:sub>50<\/jats:sub> values of citral for intramuscular injection and intragastric administration were 0.09 and 0.26\u2009g\u2009kg<jats:sup>\u22121<\/jats:sup> respectively. Citral significantly reduced cytokines (IL\u20101\u03b2, IL\u20106, TNF\u2010\u03b1) and oxidative factors (malondialdehyde and hydroxyl radicals) of MRSA\u2010infected mice, whereas it increased gluthtione and superoxide dismutase levels. Citral can reduce the lung inflammatory infiltrates infected by MRSA.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Citral exerted a dose\u2010dependent anti\u2010MRSA effect and ameliorated MRSA\u2010induced abnormal changes in inflammation and oxidative stress. This indicates that citral has the potential for development as a new anti\u2010MRSA drug. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.9677","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T02:32:15Z","timestamp":1553049135000},"page":"4423-4429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Effect and mechanism of citral against methicillin\u2010resistant <i>Staphylococcus aureus in vivo<\/i>"],"prefix":"10.1002","volume":"99","author":[{"given":"Nana","family":"Long","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Drug Residue Prevention and Control Technology of Animal\u2010Derived Food Chengdu Medical College  Chengdu PR China"},{"name":"School of Laboratory Medicine Chengdu Medical College  Chengdu PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaqiao","family":"Tang","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Drug Residue Prevention and Control Technology of Animal\u2010Derived Food Chengdu Medical College  Chengdu PR China"},{"name":"School of Laboratory Medicine Chengdu Medical College  Chengdu PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenghui","family":"Sun","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Drug Residue Prevention and Control Technology of Animal\u2010Derived Food Chengdu Medical College  Chengdu PR China"},{"name":"School of Laboratory Medicine Chengdu Medical College  Chengdu PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Drug Residue Prevention and Control Technology of Animal\u2010Derived Food Chengdu Medical College  Chengdu PR China"},{"name":"School of Laboratory Medicine Chengdu Medical College  Chengdu PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4988-9002","authenticated-orcid":false,"given":"Min","family":"Dai","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Drug Residue Prevention and Control Technology of Animal\u2010Derived Food Chengdu Medical College  Chengdu PR China"},{"name":"School of Laboratory Medicine Chengdu Medical College  Chengdu PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808203390806"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.27.1.56-71.1963"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055356"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc09-0295"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1D178"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/153857440503900404"},{"key":"e_1_2_8_8_1","first-page":"201","article-title":"Ceftaroline fosamil, a cephalosporin derivative for the potential treatment of MRSA infection","volume":"9","author":"Parish D","year":"2008","journal-title":"Curr Opin Investig Drugs"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.25"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2012.07.017"},{"key":"e_1_2_8_11_1","article-title":"Simulation of the vacuum distillation separating process of citral from \n                     Litsea cubeba\n                   oil","volume":"1","author":"Wang X","year":"2013","journal-title":"Med Plant"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","unstructured":"MaM LeiE MengH WangT XieL ShenD et al.Cluster and principal component analysis based on SSR markers of Amomum tsao\u2010ko in Jinping County of Yunnan Province. 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