{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:47:48Z","timestamp":1786978068728,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T00:00:00Z","timestamp":1556150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2019,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal\u2010tolerant bacteria from soils in olive tree fields routinely treated with copper\u2010derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Five hundred and ninety\u2010five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) \u2265 16\u2009mmol\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs \u2265 12\u2009mmol\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      for nickel. Ninety\u2010six metal\u2010tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      (37%),\n                      <jats:italic>Bacillus<\/jats:italic>\n                      (23%) and\n                      <jats:italic>Chryseobacterium<\/jats:italic>\n                      (20%), while 6% were identified as\n                      <jats:italic>Variovorax<\/jats:italic>\n                      , 4% as\n                      <jats:italic>Stenotrophomonas<\/jats:italic>\n                      and 2% as\n                      <jats:italic>Serratia<\/jats:italic>\n                      or\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      . Highest copper tolerance was detected among\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      . Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim\/sulfamethoxazole.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. \u00a9 2019 Society of Chemical Industry<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.9708","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T02:59:24Z","timestamp":1553482764000},"page":"4677-4685","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds"],"prefix":"10.1002","volume":"99","author":[{"given":"Nicol\u00e1s","family":"Glibota","sequence":"first","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00aa Jos\u00e9","family":"Grande Burgos","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5894-5029","authenticated-orcid":false,"given":"Antonio","family":"G\u00e1lvez","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ortega","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales Universidad de Ja\u00e9n  Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2009.04.026"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-014-1383-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2006.02.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2009.05.051"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/es101798r"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2017.09.084"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ampbs.2017.02.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics4040567"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-012-0726-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.04.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1220761"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01802-16"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2016.10.074"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2016.12.027"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2015.05.102"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2016.10.075"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6404-9"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2016.01.007"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13370"},{"key":"e_1_2_7_21_1","first-page":"25862","article-title":"Zinc and copper in animal feed\u00a0\u2013\u00a0development of resistance and co\u2010resistance to antimicrobial agents in bacteria of animal origin","volume":"25","author":"Yazdankhah S","year":"2014","journal-title":"Microb Ecol Health Dis"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.04283-13"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","unstructured":"Van NotenN GorissenLandDe SmetS <REFATL>Assistance in the update of the systematic literature review (SLR): \u2018Influence of copper on antibiotic resistance of gut microbiota on pigs (including piglets)\u2019.EFSA Support Publ13(3):1005E (2016).","DOI":"10.2903\/sp.efsa.2016.EN-1005"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-009-1114-3"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2004.01650.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-016-2191-4"},{"key":"e_1_2_7_27_1","first-page":"109","article-title":"The accumulation of heavy metals Pb, Cu and Cd at roadside forest soil","volume":"14","author":"Grigalavi\u010dien\u0117 I","year":"2005","journal-title":"Pol J Environ Stud"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2389.1996.tb01852.x"},{"key":"e_1_2_7_29_1","first-page":"303","article-title":"Study of heavy metal and arsenic concentrations in olive farm soils, Sierra M\u00e1gina, Jaen, Spain","volume":"58","author":"Menjivar Flores JC","year":"2009","journal-title":"Acta Agron"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj1978.03615995004200030009x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2005.01.007"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2004.01506.x"},{"key":"e_1_2_7_33_1","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2015"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.34"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2001.00161.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2012.07.040"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-016-7997-y"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027300"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222743110"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2016.11.072"},{"key":"e_1_2_7_41_1","first-page":"107","article-title":"Newer systems for bacterial resistances to toxic heavy metals","volume":"102","author":"Silver S","year":"1994","journal-title":"Environ Health Perspect"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2012.05.018"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01918-14"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.12.009"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-010-1690-2"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-018-6471-3"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.18869\/modares.iem.2.1.25"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.9708","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.9708","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.9708","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.9708","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:33:52Z","timestamp":1760870032000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.9708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,25]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,8,15]]}},"alternative-id":["10.1002\/jsfa.9708"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.9708","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,25]]},"assertion":[{"value":"2018-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}