{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:42:58Z","timestamp":1777480978680,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T00:00:00Z","timestamp":1567209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>This study was conducted to investigate effects of dietary zinc methionine (Zn\u2010Met) supplementation on laying performance, zinc (Zn) status, intestinal morphology, and Zn transporters in laying hens compared with zinc sulfate (ZnSO<jats:sub>4<\/jats:sub>). A total of 384 Hyline Grey laying hens (38\u2009weeks old) with similar performance (1.42\u2009\u00b1\u20090.07\u2009kg) were randomly allotted to four dietary treatments and fed with a basal diet (control) or the basal diet supplemented with Zn, either as Zn\u2010Met at 40 and 80\u2009mg Zn\/kilogram diet or as ZnSO<jats:sub>4<\/jats:sub> at 80\u2009mg Zn\/kilogram diet, for 10\u00a0weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>There was no difference in egg weight, egg production, feed intake, and feed conversation ratio among all groups (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). Compared with the control, Zn contents were increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in the liver, duodenum, and jejunum of laying hens fed diets supplemented with different Zn sources. There was no difference (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05) in Zn contents in liver, duodenum, and jejunum between diets supplemented with Zn\u2010Met or ZnSO<jats:sub>4<\/jats:sub> at 80\u2009mg Zn\/kilogram diet. Compared with the control and the ZnSO<jats:sub>4<\/jats:sub> group (80\u2009mg Zn\/kilogram diet), supplementation with Zn\u2010Met of 80\u2009mg Zn\/kilogram diet increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) villus height, villus area, and villus height\/crypt depth ratio but reduced (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) crypt depth in jejunum. Expression of metallothionein messenger RNA of jejunum in the group fed a diet containing Zn\u2010Met (80\u2009mg Zn\/kilogram diet) was higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) than that in the control.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results indicated that Zn\u2010Met has positive effects on the Zn status of liver, duodenum, and jejunum, intestinal morphology, and metallothionein messenger RNA expression in jejunum of laying hens compared with ZnSO<jats:sub>4<\/jats:sub>. \u00a9 2019 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.9941","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T03:33:07Z","timestamp":1563766387000},"page":"6582-6588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Effects of zinc methionine supplementation on laying performance, zinc status, intestinal morphology, and expressions of zinc transporters' mRNA in laying hens"],"prefix":"10.1002","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0728-2333","authenticated-orcid":false,"given":"Lanlan","family":"Li","sequence":"first","affiliation":[{"name":"Feed Science Institute, College of Animal Science Zhejiang University  Hangzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaiyu","family":"Li","sequence":"additional","affiliation":[{"name":"Feed Science Institute, College of Animal Science Zhejiang University  Hangzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenting","family":"Zhou","sequence":"additional","affiliation":[{"name":"Feed Science Institute, College of Animal Science Zhejiang University  Hangzhou People's Republic of 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