{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T11:07:27Z","timestamp":1774868847172,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,9,26]],"date-time":"2002-09-26T00:00:00Z","timestamp":1032998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Prior studies have shown that sarcoma growth can be stimulated by insulin\u2010like growth factor\u2010I (IGF\u2010I). To extend this line of research, we carried out in vivo growth studies of primary human sarcoma in immunosuppressed control and IGF\u2010I\u2013deficient mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human sarcoma specimens (one osteosarcoma and seven soft tissue sarcomas) were harvested in the operating room and implanted in immunosuppressed mice. Second\u2010generation sarcomas were transplanted to control (GH replete <jats:italic>lit<\/jats:italic>\/+ mice) and to experimental (GH\/IGF\u2010I\u2013deficient <jats:italic>lit<\/jats:italic>\/<jats:italic>lit<\/jats:italic>) animals. When tumors reached 1,000 mm<jats:sup>3<\/jats:sup> in one group, average tumor size was compared in the two groups. IGF\u2010I receptor expression was measured by RT\u2010PCR and IGF\u2010I receptor binding sites were assayed by radiolabeled IGF\u2010I.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five of eight sarcomas demonstrated reduced growth in the GH\/IGF\u2010I\u2013deficient <jats:italic>lit<\/jats:italic>\/<jats:italic>lit<\/jats:italic> animals. In four of the five sarcomas that demonstrated growth inhibition, IGF\u2010R was elevated relative to placenta or a positive control cell line (MCF\u20107, which is known to be responsive to IGF\u2010I in vitro and in vivo). In three of the five sarcomas that demonstrated growth suppression, IGF\u2010R was elevated twofold after implantation in the experimental IGF\u2010I\u2013deficient animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The GH\u2013IGF axis may be an important stimulator of tumor growth in sarcomas. These experiments suggest that IGF suppression may inhibit sarcoma growth in vivo. J. Surg. Oncol. 2002;81:75\u201379. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.10136","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T04:56:43Z","timestamp":1034139403000},"page":"75-79","source":"Crossref","is-referenced-by-count":24,"title":["Reduced growth of human sarcoma xenografts in hosts homozygous for the <i>lit<\/i> 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