{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:33:08Z","timestamp":1770823988712,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,23]],"date-time":"2004-02-23T00:00:00Z","timestamp":1077494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The axillary sentinel lymph node biopsy (SLNB) has gained increasing popularity as a novel surgical approach for staging patients with breast carcinoma and for guiding the choice of adjuvant therapy with minimal morbidity. Patients with negative SLNB represent a subset of breast carcinoma patients with definitely better prognosis, because their pN0 status is based on a very thorough examination of the sentinel lymph nodes (SLNs), with a very low risk of missing even small micrometastatic deposits, as compared with routine examination of the 20 or 30 lymph nodes obtained by the traditional axillary clearing. The histopathologic examination of the SLNs may be performed after fixation and embedding in paraffin, or intraoperatively on frozen sections. Whatever is the preferred tracing technique and surgical procedure, the histopathologic examination of each SLN must be particularly accurate, to avoid a false\u2010negative diagnosis. Unfortunately, because of the lack of standardised guidelines or protocols for SLN examination, different institutions still adopt their own working protocols, which differ substantially in the number of sections cut and examined, in the cutting intervals (ranging from 50 to more than 250 \u03bcm), and in the more or less extensive use of immunohistochemical assays for the detection of micrometastatic disease. Herein, a very stringent protocol for the examination of the axillary SLN is reported, which is applied either to frozen SLN for the intraoperative diagnosis, and to fixed and embedded SLN as well. J. Surg. Oncol. 2004;85:123\u2013128. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jso.20024","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T20:38:05Z","timestamp":1077827885000},"page":"123-128","source":"Crossref","is-referenced-by-count":31,"title":["Histopathologic examination of axillary sentinel lymph nodes in breast carcinoma patients"],"prefix":"10.1002","volume":"85","author":[{"given":"Giuseppe","family":"Viale","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica","family":"Sonzogni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giancarlo","family":"Pruneri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fausto","family":"Maffini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Masullo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Dell'Orto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Mazzarol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199409000-00015"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1997.15.6.2345"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199709000-00006"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)01004-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199810013391401"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/91.4.368"},{"key":"e_1_2_1_8_2","first-page":"1565","article-title":"Prognostic importance of occult axillary lymph node micrometastases from breast cancer","volume":"335","author":"International (Ludwig) Breast Cancer Study Group","year":"1990","journal-title":"Lancet"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990601)85:11<2433::AID-CNCR18>3.0.CO;2-3"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.1999.00667.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.137.10.1157"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/10520299409106292"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19971001)80:7<1188::AID-CNCR2>3.0.CO;2-H"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)11104-2"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199904000-00013"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.6.1720"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010915)92:6<1378::AID-CNCR1460>3.0.CO;2-Y"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20000801)89:3<574::AID-CNCR12>3.0.CO;2-Y"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.12.926"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19991215)86:12<2668::AID-CNCR11>3.0.CO;2-R"},{"key":"e_1_2_1_21_2","volume-title":"UICC TNM Classification of malignant tumours","author":"Sobin LH","year":"2002"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990301)85:5<1098::AID-CNCR13>3.0.CO;2-N"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990915)86:6<990::AID-CNCR14>3.0.CO;2-D"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1177\/000313489906500602","article-title":"Sentinel node biopsy and cytokeratin staining for the accurate staging of 478 breast cancer patients","volume":"65","author":"Pendas S","year":"1999","journal-title":"Am Surg"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1987.tb00189.x"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01600298"},{"key":"e_1_2_1_27_2","first-page":"4176","article-title":"Clinical significance of micrometastases in axillary lymph nodes assessed by reverse transcription\u2010polymerase chain reaction in breast cancer patients","volume":"6","author":"Masuda N","year":"2000","journal-title":"Clin Cancer Res"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.8.2632"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19981218)79:6<645::AID-IJC16>3.0.CO;2-R"},{"key":"e_1_2_1_30_2","first-page":"1147","article-title":"RT\u2010PCR detection of breast cancer cells in sentinel lymph nodes","volume":"16","author":"Kataoka A","year":"2000","journal-title":"Int J Oncol"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20010920)95:5<307::AID-IJC0153>3.0.CO;2-Q"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1309\/7EF8-F1W7-YVNT-H8H5"},{"key":"e_1_2_1_33_2","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.5858\/2001-125-1497-APSOFP","article-title":"A potential source of false\u2010positive sentinel nodes. Immunostain misadventure","volume":"125","author":"Chiu A","year":"2001","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200304000-00012"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/59.5.666"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.245"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02029.x"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1980.tb02958.x"}],"container-title":["Journal of Surgical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjso.20024","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjso.20024","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jso.20024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T23:21:39Z","timestamp":1700176899000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jso.20024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,23]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["10.1002\/jso.20024"],"URL":"https:\/\/doi.org\/10.1002\/jso.20024","archive":["Portico"],"relation":{},"ISSN":["0022-4790","1096-9098"],"issn-type":[{"value":"0022-4790","type":"print"},{"value":"1096-9098","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2,23]]}}}