{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T22:06:59Z","timestamp":1773180419746,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T00:00:00Z","timestamp":1259539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>This retrospective study was performed to evaluate the status of p53 in pleomorphic adenomas and carcinomas ex pleomorphic adenoma in the parotid gland. As loss or mutation of p53 can cause malignant transformation, the possible degeneration of pleomorphic adenomas to carcinomas ex pleomorhic adenoma was investigated by mutational analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010five Patients including 14 patients with pleomorphic adenomas and 11 patients with carcinoma ex pleomorphic adenoma of the parotid gland were examined for p53 status. DNA was extracted out of paraffin\u2010embedded tissue and PCR was performed for the coding exons 2\u201311. Denaturing gradient gel electrophoresis (DGGE) was carried out for mutational analysis and DNA sequencing was performed in case of a suspected mutation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fourteen pleomorphic adenomas and 11 carcinomas ex pleomorphic adenoma were screened for p53 status and potent mutations. Subsequent sequencing of the distinct exons showed no mutation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We could not detect mutations of p53 neither in benign nor malignant parotid tumors and we therefore assume that p53 plays no role in the transformation from pleomorphic adenoma to carcinoma ex pleomorphic adenoma. J. Surg. Oncol. 2010;101:127\u2013130. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.21444","type":"journal-article","created":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T21:26:56Z","timestamp":1259616416000},"page":"127-130","source":"Crossref","is-referenced-by-count":14,"title":["Transformation of pleomorphic adenoma to carcinoma ex pleomorphic adenoma of the parotid gland is independent of p53 mutations"],"prefix":"10.1002","volume":"101","author":[{"given":"Claudia","family":"Gedlicka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chike B.","family":"Item","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"W\u00f6gerbauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helga","family":"Martinek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregor","family":"Heiduschka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boban M.","family":"Erovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthaeus","family":"Ch.Grasl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietmar","family":"Thurnher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,15]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"473","volume-title":"Praxis der HNO\u2010Heilkunde, Kopf\u2010 und Halschirurgie","author":"Strutz J","year":"2001"},{"key":"e_1_2_1_3_2","first-page":"1041","article-title":"Upregulation of Igf and Wnt singnalling associated genes in pleomorphic adenomas of the salivary glands in PLAG1 transgenic mice","volume":"32","author":"Declercq J","year":"2008","journal-title":"Int J Oncol"},{"key":"e_1_2_1_4_2","first-page":"477","article-title":"Expression and mutation patterns of p53 in benign and malignant salivary gland tumors","volume":"16","author":"Nordkvist A","year":"2000","journal-title":"Int J Oncol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/106689690501300405"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.5.1379"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00115.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/18.3.299"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19970615)79:12<2314::AID-CNCR4>3.0.CO;2-L"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1997.tb01227.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-64402005000200014"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2005.02157.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1997)9:4<348::AID-HUMU8>3.0.CO;2-1"},{"key":"e_1_2_1_14_2","first-page":"833","article-title":"p53 mutation\/p53\u2010protein over expression","volume":"47","author":"Bosch FX","year":"1999","journal-title":"Differential significance for the progression of head\u2010neck carcinomas. 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