{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:36:25Z","timestamp":1760708185316},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,28]],"date-time":"2010-04-28T00:00:00Z","timestamp":1272412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Microarray\u2010CGH facilitates analysis of cancer\u2010associated genomic differences between normal and tumor tissues and provides a genome\u2010wide assessment of copy number variations (CNVs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To identify CNVs and their clinical significance in gastric cancer, Microarray\u2010CGH was performed to identify CNVs with genomic DNA (gDNA) from normal placenta tissue, peripheral blood mononuclear cells (PBMCs), and normal gastric tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 20 CNVs, including 8 novel CNVs, were identified by Microarray\u2010CGH. Among the 20 CNVs, 5 showed an aberration frequency of over 50%. In addition, mRNA expression of W72437 (TFIIH), AI968311 (GAGE10), AI352361, and AA169807 (PTCH1) in normal tissues and AA485362 (GPX1), AI201652, and AI968311 (GAGE10) in cancer tissues was associated with DNA change. As a whole, incidences of oncogene\u2010like, suppressor\u2010like, and innocent CNVs were 13.8%, 13.2%, and 73.0%, respectively (gain 11.4%, loss 11.8%). AA936795 (C19orf61) appeared as an oncogene\u2010like CNV (9\/30, 30%), A1352361 (13\/30, 43%), and AA281797 (LOC728340, 10\/30, 33%) appeared as tumor suppressor\u2010related CNVs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study identified gastric cancer\u2010associated and innocent CNVs in gDNA isolated from placenta tissue and PBMC, which are generally used as reference samples in Microarray\u2010CGH. These novel CNVs may be used for gastric cancer\u2010specific gene selection in comparative analysis of genomics. J. Surg. Oncol. 2010;102:454\u2013461. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.21585","type":"journal-article","created":{"date-parts":[[2010,4,29]],"date-time":"2010-04-29T14:17:05Z","timestamp":1272550625000},"page":"454-461","source":"Crossref","is-referenced-by-count":11,"title":["Identification of novel gastric cancer\u2010associated CNVs by integrated analysis of 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