{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:31:09Z","timestamp":1791145869038,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,8,23]],"date-time":"2012-08-23T00:00:00Z","timestamp":1345680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2013,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The destruction of the basement membrane (BM) is the first step in cancer invasion and metastasis. Type IV collagen is a major component of the BM, and is composed of six genetically distinct \u03b1(IV) chains; \u03b11(IV) to \u03b16(IV). The loss of \u03b15(IV) and \u03b16(IV) chains from the epithelial BM at the early stage of cancer invasion has been reported in several types of cancers. However, the expression of \u03b15(IV) and \u03b16(IV) chains in extrahepatic bile duct carcinoma (EBDC) remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the expression of \u03b1(IV) chains by immunohistochemistry using 71 resected EBDC specimens. Prognostic significance of \u03b1(IV) chains was examined by Cox regression and Kaplan\u2013Meier analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the invasive cancer, the expression of \u03b16(IV) chain in the BM was lost partially or completely preceded by the loss of \u03b12(IV) chain. The loss of \u03b16(IV) chain in the BM of the invasive cancer was related to the tumor classification, TNM stages, and the expression of \u03b12(IV) chain. The patients with \u03b12(IV)\u2010negative and \u03b16(IV)\u2010negative chains had significantly poorer prognosis than those with \u03b12(IV)\u2010positive and \u03b16(IV)\u2010positive\/negative chains (<jats:italic>P<\/jats:italic>\u2009=\u20090.04).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The loss of \u03b12(IV) and \u03b16(IV) chains might be a useful prognostic factor in patients with EBDC. J. Surg. Oncol. 2013;107:402\u2013407. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.23225","type":"journal-article","created":{"date-parts":[[2012,8,23]],"date-time":"2012-08-23T17:29:24Z","timestamp":1345742964000},"page":"402-407","source":"Crossref","is-referenced-by-count":12,"title":["Differential expression of basement membrane type IV collagen \u03b12 and \u03b16 chains as a prognostic factor in patients with extrahepatic bile duct carcinoma"],"prefix":"10.1002","volume":"107","author":[{"given":"Kotaro","family":"Hirashima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken\u2010Ichi","family":"Iyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshifumi","family":"Baba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumi","family":"Honda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Sado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshifumi","family":"Ninomiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Takamori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Beppu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Baba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,23]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"636","article-title":"Tumor invasion and the extracellular matrix","volume":"49","author":"Liotta LA","year":"1983","journal-title":"Lab Invest"},{"key":"e_1_2_6_3_2","first-page":"1","volume-title":"Molecular and cellular aspects of basement membrane","author":"Roahrbach D","year":"1993"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1094"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(90)90014-R"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022003"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"23013","DOI":"10.1016\/S0021-9258(17)31612-5","article-title":"Complete primary structure of the human alpha 3(IV) collagen chain. 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