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Pharyngocutaneous fistula is a common complication of laryngectomy. We hypothesized that decellularized and lyophilized human amnion\/chorion membrane can be an effective, non\u2010invasive method of treating pharyngocutaneous fistula.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 67 patients with laryngeal squamous cell carcinoma were retrospectively analyzed after treatment in a prospective trial. After preoperative chemotherapy, radiotherapy, and total or extended laryngectomy, primary wound healing occurred in 42 (62.7%) patients. Pharyngocutaneous fistula developed in 8 (11.9%) patients. Decellularized and lyophilized human amnion\/chorion membrane grafts were used to reconstruct the fistulas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average time for the full healing of the wound in all patients after transplantation of these grafts was 18 days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The advantages of using these grafts over other existing methods of pharyngocutaneous fistula treatment are that they are non\u2010invasive, prevent donor morbidity, and enable management of the wound without using classical wound gauze. <jats:italic>J. Surg. Oncol. 2016;113:538\u2013543<\/jats:italic>. \u00a9 2016 The Authors. <jats:italic>Journal of Surgical Oncology<\/jats:italic> Published by Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.24163","type":"journal-article","created":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T06:24:05Z","timestamp":1453357445000},"page":"538-543","source":"Crossref","is-referenced-by-count":32,"title":["Clinical application of decellularized and lyophilized human amnion\/chorion membrane grafts for closing post\u2010laryngectomy pharyngocutaneous fistulas"],"prefix":"10.1002","volume":"113","author":[{"given":"Zurab","family":"Kakabadze","sequence":"first","affiliation":[{"name":"Tbilisi State Medical University Tbilisi Georgia"},{"name":"Cancer Research Center Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantine","family":"Mardaleishvili","sequence":"additional","affiliation":[{"name":"Tbilisi State Medical University Tbilisi Georgia"},{"name":"Cancer Research Center Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Loladze","sequence":"additional","affiliation":[{"name":"Cancer Research Center Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivane","family":"Javakhishvili","sequence":"additional","affiliation":[{"name":"Cancer Research Center Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantine","family":"Chakhunasvili","sequence":"additional","affiliation":[{"name":"Tbilisi State Medical University Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lika","family":"Karalashvili","sequence":"additional","affiliation":[{"name":"Tbilisi State Medical University Tbilisi Georgia"},{"name":"Ilia State University Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natia","family":"Sukhitashvili","sequence":"additional","affiliation":[{"name":"Ilia State University Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gocha","family":"Chutkerashvili","sequence":"additional","affiliation":[{"name":"Ilia State University Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Kakabadze","sequence":"additional","affiliation":[{"name":"Tbilisi State Medical University Tbilisi Georgia"},{"name":"Ilia State University Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Chakhunasvili","sequence":"additional","affiliation":[{"name":"Tbilisi State Medical University Tbilisi Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,21]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Cancer research UK http:\/\/www.cancerresearchuk.org\/health\u2010professional\/cancer\u2010statistics\/statistics\u2010by\u2010cancer\u2010type\/laryngeal\u2010cancer#heading\u2010Zero Sept2015."},{"key":"e_1_2_6_3_1","unstructured":"SEER cancer statistics factsheets: Larynx cancer. 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