{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T06:02:58Z","timestamp":1771999378245,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mesothelin is a cell surface glycoprotein overexpressed in 28%\u201358% of colorectal cancer (CRC). We hypothesized that CRC mesothelin expression contributes to peritoneal spread and that it is selectively overexpressed in those with peritoneal metastasis versus distant metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This case\u2013controlled study involved mesothelin immunohistochemistry staining of tumor specimens from patients with metastatic CRC\/appendiceal cancers between 2017 and 2019. Staining reactivity was graded from trace to 4+ (low \u22641+; high &gt;1+). Staining patterns were characterized on global (focal\/patchy\/diffuse) and cellular (apical\/cytoplasmic) levels. Immunostaining of normal mesothelial cells served as internal control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirty\u2010one patients were identified: 11 peritoneal (study) and 20 distant metastasis (control). The control group did not include appendiceal cancers. The study group had greater proportion of high staining reactivity (55% vs. 5%; odds ratio [OR]\u2009=\u200920.4, 95% confidence interval [CI] 1.96\u2013211.8). The study group had more diffuse (36% vs. 0%; OR\u2009=\u200922.2, 95% CI 1.1\u2013465.3) and cytoplasmic staining patterns (73% vs. 28%; OR\u2009=\u20096.9, 95% CI 1.3\u201337.2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mesothelin expression is higher in CRC\/appendiceal cancers with peritoneal metastasis than those with distant metastasis. Immunohistochemistry staining patterns suggestive of propensity towards peritoneal metastasis include diffuse and cytoplasmic staining. Mesothelin may be a potential target for novel treatments of CRC\/appendiceal carcinoma with peritoneal involvement.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.26610","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T17:41:19Z","timestamp":1627321279000},"page":"1091-1097","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prevalence of mesothelin expression in peritoneal disease from colorectal and appendiceal cancers"],"prefix":"10.1002","volume":"124","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0491-112X","authenticated-orcid":false,"given":"Dong H.","family":"Yoon","sequence":"first","affiliation":[{"name":"Division of Colorectal Surgery, Department of Surgery, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Pathology, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei","family":"Tatishchev","sequence":"additional","affiliation":[{"name":"Department of Pathology, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjun P. N.","family":"Duldulao","sequence":"additional","affiliation":[{"name":"Division of Colorectal Surgery, Department of Surgery, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang W.","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Colorectal Surgery, Department of Surgery, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joongho","family":"Shin","sequence":"additional","affiliation":[{"name":"Division of Colorectal Surgery, Department of Surgery, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"American Cancer Society: Cancer Facts and Figures 2020. Atlanta Ga: American Cancer Society. 2020.https:\/\/www.cancer.org\/content\/dam\/cancer-org\/research\/cancer-facts-and-statistics\/annual-cancer-facts-and-figures\/2020\/cancer-facts-and-figures-2020.pdf. 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