{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T18:09:54Z","timestamp":1769105394030,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T00:00:00Z","timestamp":1644624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472677"],"award-info":[{"award-number":["81472677"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070655"],"award-info":[{"award-number":["82070655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The clinicopathological and prognostic significance of human epidermal growth factor receptor 2 (HER2) status in surgically resected colorectal liver metastases (CRLM) remains uncertain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HER2 expression was evaluated by immunohistochemical (IHC) in two CRLM tissue microarrays (TMAs). For samples with an IHC score of 2+ or 3+, fluorescence in situ hybridization (FISH) was performed to assess HER2 amplification. The association of HER2 amplification with clinicopathological parameters and prognosis was assessed using Fisher's exact test and Kaplan\u2013Meier method, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HER2 expression was consistent between primary tumor and liver metastases in 66.9% (85\/127) cases (<jats:italic>r<\/jats:italic>\u2009=\u20090.643, <jats:italic>p<\/jats:italic>\u2009=\u20090.001). After FISH validation, HER2 amplification was identified in 6.25% (13\/208) patients. HER2 amplification was significantly associated with age (<jats:italic>p<\/jats:italic>\u2009=\u20090.017), bilobar involvement (<jats:italic>p<\/jats:italic>\u2009=\u20090.005) and left\u2010sided RAS\/RAF wild\u2010type status (<jats:italic>p<\/jats:italic>\u2009=\u20090.002). In the overall cohort, HER2 amplification was correlated with significantly worse relapse\u2010free survival (RFS). Further stratification revealed that among left\u2010sided RAS\/RAF wild\u2010type cases, HER2 amplification was significantly associated with worse overall survival (OS) (30.2 vs. 50.9 months, <jats:italic>p<\/jats:italic>\u2009=\u20090.040) and RFS (5.77 vs. 19.97 months, <jats:italic>p<\/jats:italic>\u2009=\u20090.017).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HER2 amplification is more enriched in CRLMs with younger age, left\u2010sided RAS\/RAF wild\u2010type, and bilobar involvement. Moreover, HER2 amplification predicts a poorer prognosis especially in left\u2010sided RAS\/RAF wild\u2010type CRLMs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.26815","type":"journal-article","created":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T16:19:02Z","timestamp":1644682742000},"page":"991-1001","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Clinicopathological and prognostic significance of HER2 status in surgically resected colorectal liver metastases"],"prefix":"10.1002","volume":"125","author":[{"given":"Jiahao","family":"Han","sequence":"first","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaochen","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yitong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenmei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huliang","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jubo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-015-9719-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.25421"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i28.6345"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(89)90343-1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25120"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61121-X"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-019-0268-3"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.98"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdy100"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)00150-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(18)30904-5"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(21)00086-3"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-2012-00282"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2018-0152"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdy156"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v11.i4.335"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clcc.2018.05.006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.0106"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098528"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2006.038281"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.121"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11599"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2001.01084.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-018-2744-z"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4679"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70120-3"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000011911"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/109063"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/CAD.0000000000000510"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14022370"}],"container-title":["Journal of Surgical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jso.26815","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jso.26815","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jso.26815","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T20:30:35Z","timestamp":1692995435000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jso.26815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,12]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/jso.26815"],"URL":"https:\/\/doi.org\/10.1002\/jso.26815","archive":["Portico"],"relation":{},"ISSN":["0022-4790","1096-9098"],"issn-type":[{"value":"0022-4790","type":"print"},{"value":"1096-9098","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,12]]},"assertion":[{"value":"2021-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}