{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T19:39:36Z","timestamp":1775590776892,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Penile cancer is high in some underdeveloped countries. Signal transducer and activator of transcription 3 (STAT3) and CD44, CD24, and SOX2+ are known to be markers of diagnosis and prognosis in other cancers, but without studies in penile cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross\u2010sectional study was conducted at the Hospital de Cancer de Pernambuco from March 2015 to December 2017. We performed SOX2, STAT3, CD24, and CD44 analyses in blood and tumor tissue by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High levels of CD44<jats:sup>high<\/jats:sup>CD24<jats:sup>low<\/jats:sup>, CD44<jats:sup>high<\/jats:sup>CD24<jats:sup>low<\/jats:sup>pSTAT3+ and CD44<jats:sup>hig<\/jats:sup><jats:sup>h<\/jats:sup>CD24<jats:sup>low<\/jats:sup> in the blood of patients compared to the controls (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Low of SOX2+\u2009T cells in blood of patients compared to controls. High CD44<jats:sup>high<\/jats:sup>CD24<jats:sup>low<\/jats:sup> levels in patients with perineural invasion (PNI), tumor size\u2009&gt;\u20093\u2009cm, and pT2 stage (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). High T cell levels in the blood and tumor tissue of patients with tumor \u22643\u2009cm (<jats:italic>p<\/jats:italic> &lt; 0.05). Increased SOX2+\u2009T cells in blood of patients with PNI (\u2010) and pT1 stage (<jats:italic>p<\/jats:italic> &lt; 0.05). CD44<jats:sup>high<\/jats:sup>CD24l<jats:sup>ow<\/jats:sup>pSTAT3+\u2009(<jats:italic>r<\/jats:italic>\u2009=\u20090.669; <jats:italic>p<\/jats:italic>\u2009=\u20090.024) and SOX2+T cells (<jats:italic>r<\/jats:italic>\u2009=\u20090.404, <jats:italic>p<\/jats:italic>\u2009=\u20090.029) correlation were observed between blood and tumor tissue in penile cancer patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD44, CD24, and SOX2 molecules were markers of advanced disease associated with the worst prognosis in CaPe. However, pSTAT3 and T cells were associated with a more favorable prognosis in this study.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.27749","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T07:28:45Z","timestamp":1724052525000},"page":"929-943","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression of CD44<sup>high<\/sup>CD24<sup>Low<\/sup> cells, SOX2, and STAT3 transcription factors on peripheral blood and tumor tissue of penile squamous cell carcinoma"],"prefix":"10.1002","volume":"130","author":[{"given":"Felipe Dubourcq","family":"de Barros","sequence":"first","affiliation":[{"name":"Translational Research Laboratory Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) Recife Brazil"},{"name":"Hospital de C\u00e2ncer de Pernambuco Recife Brazil"},{"name":"Postgraduate Program in Translational Medicine Universidade Federal de S\u00e3o Paulo (UNIFESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo Cardoso","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"AC Camargo Cancer Center S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8705-8210","authenticated-orcid":false,"given":"M\u00e1rio Rino","family":"Martins","sequence":"additional","affiliation":[{"name":"Translational Research Laboratory Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) Recife Brazil"},{"name":"Hospital de C\u00e2ncer de Pernambuco Recife Brazil"},{"name":"Postgraduate Program in Translational Medicine Universidade Federal de S\u00e3o Paulo (UNIFESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe da Silva","family":"Marinho","sequence":"additional","affiliation":[{"name":"Translational Research Laboratory Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) Recife Brazil"},{"name":"Hospital de C\u00e2ncer de Pernambuco Recife Brazil"},{"name":"AC Camargo Cancer Center S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Augusto","family":"Soares","sequence":"additional","affiliation":[{"name":"AC Camargo Cancer Center S\u00e3o Paulo Brazil"},{"name":"Rede D'Or S\u00e3o Luiz S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leuridan Cavalcante","family":"Torres","sequence":"additional","affiliation":[{"name":"Translational Research Laboratory Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) Recife Brazil"},{"name":"Hospital de C\u00e2ncer de Pernambuco Recife Brazil"},{"name":"Postgraduate Program in Translational Medicine Universidade Federal de S\u00e3o Paulo (UNIFESP)  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"171","volume-title":"Urologia fundamental","author":"Pompeo A","year":"2010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.57167\/Rev-SBPH.12.463"},{"key":"e_1_2_9_4_1","article-title":"Incidence of penile cancer worldwide: systematic review and meta\u2010analysis","volume":"41","author":"Montes Cardona CE","year":"2017","journal-title":"Rev Panam Salud Publica"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.04.028"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1677-5538.IBJU.2014.06.04"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.58.062105.204854"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2010.04.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2015.639"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12155"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-017-2167-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5332.1630"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8197455"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.12.003"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0605-595"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7541555"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2734"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/708036"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2015.05.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2011.03.026"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-98802009000100018"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0530291100"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3181d5e554"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12159"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21388"},{"issue":"3","key":"e_1_2_9_26_1","first-page":"69","article-title":"Statistics corner: a guide to appropriate use of correlation coefficient in medical research","volume":"24","author":"Mukaka MM","year":"2012","journal-title":"Malawi Med\u00a0J"},{"issue":"1","key":"e_1_2_9_27_1","first-page":"19","article-title":"Phenotypic subpopulations of metastatic colon cancer stem cells: genomic analysis","volume":"6","author":"Botchkina IL","year":"2009","journal-title":"Cancer Genomics Prot"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-1034"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014062"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-010-0999-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2016.02.028"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2509-5"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2010.0053"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3497"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.suronc.2014.07.003"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-753"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1515\/raon-2015-0027"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4323"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1325"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm976"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2510"},{"issue":"12","key":"e_1_2_9_42_1","first-page":"5010","article-title":"Prognostic significance of SOX2 in head and neck cancer: a meta\u2010analysis","volume":"7","author":"Dong Z","year":"2014","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7887"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1677-55382008000500007"}],"container-title":["Journal of Surgical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jso.27749","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T05:16:54Z","timestamp":1732252614000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jso.27749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,19]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/jso.27749"],"URL":"https:\/\/doi.org\/10.1002\/jso.27749","archive":["Portico"],"relation":{},"ISSN":["0022-4790","1096-9098"],"issn-type":[{"value":"0022-4790","type":"print"},{"value":"1096-9098","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,19]]},"assertion":[{"value":"2024-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}