{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T01:44:30Z","timestamp":1781919870856,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["IG 27619"],"award-info":[{"award-number":["IG 27619"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Few preclinical models of pseudomyxoma peritonei (PMP) have been developed, probably due to the tumor's low incidence and its peculiar characteristics of slow growth. Therefore, there is a need to develop more refined PMP models that better reflect its characteristics. The aim of the study is to develop a culture strategy to generate organoid models derived from PMP patient samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We followed a strategy based on combinatorial culture conditions that include the different factors essential for PMP growth and that mimic the microenvironment present in the patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We cultured PMP samples in the presence of the various factors produced by the niche environment of PMP. We obtained 12 PMP organoid models, each of which grows under specific culture conditions. PMP\u2010derived organoids show long\u2010term expansion capacity and reproduce the genetic landscape and histological phenotype of the tumor of origin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The organoids we developed faithfully reproduce the key features of PMP disease and will allow us to understand the biology of PMP. With them, we will be able to identify key regulatory networks that support PMP progression, providing a platform for multilevel preclinical testing, identify novel diagnostic biomarkers, and generate novel targets for patient treatments.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.27850","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T09:29:43Z","timestamp":1727947783000},"page":"1213-1224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A combinatorial culture strategy to develop pseudomyxoma peritonei organoid models"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0273-5807","authenticated-orcid":false,"given":"Luca","family":"Varinelli","sequence":"first","affiliation":[{"name":"Molecular Epigenomics Unit, Department of Experimental Oncology Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia","family":"Di Bella","sequence":"additional","affiliation":[{"name":"Molecular Epigenomics Unit, Department of Experimental Oncology Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8194-9810","authenticated-orcid":false,"given":"Marcello","family":"Guaglio","sequence":"additional","affiliation":[{"name":"Peritoneal Surface Malignancies Unit, Department of Surgery Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Battistessa","sequence":"additional","affiliation":[{"name":"Molecular Epigenomics Unit, Department of Experimental Oncology Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Pisati","sequence":"additional","affiliation":[{"name":"Cogentech Ltd. Benefit Corporation with a Sole Shareholder Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommaso","family":"Cavalleri","sequence":"additional","affiliation":[{"name":"Peritoneal Surface Malignancies Unit, Department of Surgery Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Milione","sequence":"additional","affiliation":[{"name":"1st Pathology Division, Department of Phatology and Laboratory Medicine Fondazione IRCCS Istituto Nazionale dei Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Mart\u00ednez\u2010Quintanilla","sequence":"additional","affiliation":[{"name":"Translational Program, Stem Cells and Cancer Laboratory, Vall d'Hebron Institute of Oncology (VHIO) Vall d'Hebron Barcelona Hospital Campus Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick T.","family":"Caswell","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell\u2010Matrix Research, Manchester Academic Health Science Centre University of Manchester Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Baratti","sequence":"additional","affiliation":[{"name":"Peritoneal Surface Malignancies Unit, Department of Surgery Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeki","family":"Kusamura","sequence":"additional","affiliation":[{"name":"Peritoneal Surface Malignancies Unit, Department of Surgery Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Deraco","sequence":"additional","affiliation":[{"name":"Peritoneal Surface Malignancies Unit, Department of Surgery Fondazione IRCCS Istituto Nazionale Tumori di Milano Milan 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