{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T12:52:32Z","timestamp":1720961552253},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":9696,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[1980,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Breast cancers from 53 patients were explanted in culture, and 39 with two\u2010year or more follow\u2010up and documentable tissue forms of cancer in culture (histoculture) are the subject of this report. Clinicopathologic findings have been correlated with dynamic aspects of the cancers in short\u2010term histocultures, including cell cohesiveness, yield and survival time of the cancer cells in culture, emigration of macrophages from the explants, and the occurrence of special interactions of lymphocytes with cancer cells.<\/jats:p><jats:p>The outstanding finding was the association of special lymphocyte\u2010cancer cell interactions with a more favorable prognosis; six of seven patients (86%) with infiltrating ductal cancer showing lymphocyte congregation, emperiopolesis, or other special activity were tumor\u2010free survivors. Additional findings were: (1) patients having four or fewer lymph nodes positive for cancer had a survival rate of 50%, but this fell to 20% when emigrating macrophages were <jats:italic>not<\/jats:italic> present in the cancer cultures; (2) eight of 12 patients (67%) having discoid histoculture survival time of less than one week were tumor\u2010free, as opposed to three of eight patients (27%) where tumor survival in vitro was greater than one week. The reverse was true for nondiscoid, poorly cohesive colonies of cancer; (3) the combination of <jats:italic>tumor\u2010negative lymph nodes and low in vitro cancer yield<\/jats:italic> was associated with the best patient survival rate, 64%. However, the combination of negative nodes and high in vitro yield was linked to the worst patient survival (20%).<\/jats:p><jats:p>Although the overall study does not permit definitive conclusions, there is an undeniable potential for the use of histocultures in the evaluation of human cancers. Expanded studies are warranted, including larger numbers of tumors and cultures, and longer patient follow\u2010up periods.<\/jats:p>","DOI":"10.1002\/jso.2930130103","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T06:34:25Z","timestamp":1171953265000},"page":"9-20","source":"Crossref","is-referenced-by-count":17,"title":["Histoculture of human breast cancers"],"prefix":"10.1002","volume":"13","author":[{"given":"R. P.","family":"Sherwin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Richters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. E.","family":"Yellin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Donovan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"1520","article-title":"The behavior of lymphocytes in primary explants of human lung cancer in vitro","volume":"13","author":"Richters A","year":"1964","journal-title":"Lab Invest"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197412)34:6<1943::AID-CNCR2820340613>3.0.CO;2-T"},{"key":"e_1_2_1_4_2","first-page":"1111","article-title":"The pathobiologic nature of lymphocyte interactions with human breast cancer","volume":"43","author":"Sherwin RP","year":"1972","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_1_5_2","first-page":"379","volume-title":"Pathology Annual","author":"Sherwin RP","year":"1973"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(77)90012-8"}],"container-title":["Journal of Surgical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjso.2930130103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jso.2930130103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T03:27:42Z","timestamp":1699846062000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jso.2930130103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,1]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1980,1]]}},"alternative-id":["10.1002\/jso.2930130103"],"URL":"https:\/\/doi.org\/10.1002\/jso.2930130103","archive":["Portico"],"relation":{},"ISSN":["0022-4790","1096-9098"],"issn-type":[{"value":"0022-4790","type":"print"},{"value":"1096-9098","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,1]]}}}