{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T10:12:47Z","timestamp":1761559967804},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":4551,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mucin\u2010producing tumors of the pancreas (MPT) are characterized by the prodcution of much mucin and a benign course after surgical treatment. We examined 16 cases of MPT and 20 cases of \u201ccommon\u201d pancreatic duct cell carcinomas (DCC) in regard to K\u2010<jats:italic>ras<\/jats:italic> and p53 mutations. The mutations were detected by constant denaturant gel electrophoresis in combination with other techniques using PCR products amplified from the samples microdissected from the tissue sections. K\u2010<jats:italic>ras<\/jats:italic> codon 12 mutations were identified in all MPT and in 95% of DCC. On the other hand, p53 mutations were found in four of 20 (20%) DCC, and p53 was immunocy\u2010tochemically overexpressed in 3 of the 4 mutated cases. However, no p53 mutations and no p53 overexpression were identified in the 16 MPT. These results indicate that, although the K\u2010<jats:italic>ras<\/jats:italic> codon 12 mutations may be almost essential for the development of both MPT and DCC, p53 mutations seemed to be involved mainly to the latters. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jso.2930550205","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T13:15:46Z","timestamp":1171977346000},"page":"84-91","source":"Crossref","is-referenced-by-count":52,"title":["Frequent K\u2010<i>ras<\/i> mutations and absence of p53 mutations in mucin\u2010producing tumors of the pancreas"],"prefix":"10.1002","volume":"55","author":[{"given":"Tomokazu","family":"Hoshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Imai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiro","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"348","article-title":"Four cases of mucous\u2010secreting pancreatic cancer","volume":"20","author":"Ohhashi K","year":"1982","journal-title":"Prog Dig Endosc"},{"key":"e_1_2_1_3_2","first-page":"111","article-title":"Report of round table discussion on \u201cmucin\u2010producing pancreatic tumors","volume":"3","author":"Katoh H","year":"1988","journal-title":"Jpn J Pane 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