{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:56:42Z","timestamp":1790733402962,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Surgical Oncology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sentinel lymph node biopsy (SLNB) is crucial for staging and managing melanoma, but selecting patients for SLNB is challenging, with around 80% of procedures yielding negative results. The clinicopathological and gene expression profile model (CP\u2010GEP) was developed to identify low\u2010risk melanoma patients who may forgo SLNB. CP\u2010GEP combines Breslow thickness, patient age, and a gene expression analysis to classify patients as high\u2010 or low\u2010risk for nodal metastasis. This study aimed to validate the performance of CP\u2010GEP in a multicenter Danish cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Primary melanoma tissue from 536 T1\u2010T3 patients who had undergone SLNB was retrospectively analyzed using CP\u2010GEP. Results were compared with SLNB status and the Melanoma Institute Australia nomogram (MIA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>T1, T2, and T3 melanomas comprised 32.8%, 46.8%, and 20.3% of cases, respectively. The SLNB positivity rate was 18.1%. Overall, 40.9% was classified as CP\u2010GEP low\u2010risk (NPV 91.3%). Among T1 and T2 subgroups, 72.7% and 35.5% were low\u2010risk, with NPVs of 94.5% and 87.6%, respectively. For 507 patients with MIA scores, CP\u2010GEP identified 42.4% as low\u2010risk (NPV 91.2%) versus 8.1% by MIA (NPV 95.1%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CP\u2010GEP is a promising tool for supporting deselection of SLNB in melanoma patients, with a potential reduction rate of over 40%.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jso.70035","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T09:56:17Z","timestamp":1751882177000},"page":"447-455","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Validation of a Clinicopathologic and Gene Expression Model for Predicting Sentinel Node Metastasis in Melanoma: A Multicenter Danish Cohort Study"],"prefix":"10.1002","volume":"132","author":[{"given":"Marie B.","family":"Weitemeyer","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery Copenhagen University Hospital \u2013 Herlev and Gentofte Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neel M.","family":"Helvind","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery Copenhagen University Hospital \u2013 Herlev and Gentofte Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siri","family":"Klausen","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital \u2013 Herlev and Gentofte Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Clasen\u2010Linde","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital \u2013 Herlev and Gentofte Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grethe","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery and Burns Treatment Copenhagen University Hospital \u2013 Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette H.","family":"Chakera","sequence":"additional","affiliation":[{"name":"AGATA Private Hospital Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisbet R.","family":"H\u00f6lmich","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery Copenhagen University Hospital \u2013 Herlev and Gentofte Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21409"},{"key":"e_1_2_11_3_1","unstructured":"Cancer Council Australia Melanoma Guidelines Working Party. Clinical Practice Guidelines for the Diagnosis and Management of Melanoma 2020 https:\/\/www.cancer.org.au\/clinical\u2010guidelines\/skin\u2010cancer\/melanoma(accessed March 11 2025)."},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2024.0036"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2024.115153"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2016.06.407"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039\u20106060(03)00275\u20107"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.26723"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2018.12.030"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.25208"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa1310460"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.02362"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2022.0160"},{"key":"e_1_2_11_14_1","first-page":"591","article-title":"Vital Signs: Melanoma Incidence and Mortality Trends and Projections\u2014United States, 1982\u20132030","volume":"64","author":"Guy GP Jr","year":"2015","journal-title":"MMWR. 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