{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:02:08Z","timestamp":1771660928049,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81441058"],"award-info":[{"award-number":["81441058"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7212117"],"award-info":[{"award-number":["7212117"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JOR Spine"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) has been found to act as an important gasotransmitter to regulate cell activities. This study aimed to investigate the effect of H<jats:sub>2<\/jats:sub>S on autophagy of nucleus pulposus (NP) cells under hypoxia and possible mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>NP cells were isolated from rat caudal discs. Cobalt chloride was used to mimic hypoxia, sodium hydrosulfide was used to emulate exogenous H<jats:sub>2<\/jats:sub>S and 3\u2010methyladenine was used to block cell autophagy. Cell viability was assessed by phase contrast microscope and Cell Counting Kit\u20108 method. Moreover, expression of key autophagic proteins was analyzed via western blotting, and transmission electron microscopy was performed to detect autophagosomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hypoxia markedly impaired NP cell proliferation compared with control. Whereas H<jats:sub>2<\/jats:sub>S provided pro\u2010proliferation and pro\u2010autophagy effects on hypoxic NP cells. However, these beneficial impact of H<jats:sub>2<\/jats:sub>S on hypoxic NP cells were reversed by autophagy inhibitor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results showed that H<jats:sub>2<\/jats:sub>S played a cytoprotective role in NP cells exposed to hypoxia in an autophagy\u2010dependent manner.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsp2.1181","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T08:44:04Z","timestamp":1638953044000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia"],"prefix":"10.1002","volume":"4","author":[{"given":"Lei","family":"Yue","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Peking University First Hospital, Peking University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongkai","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics General Hospital of Southern Theatre Command of PLA  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoyong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Peking University First Hospital, Peking University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longtao","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Peking University First Hospital, Peking University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4938-9198","authenticated-orcid":false,"given":"Haolin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Peking University First Hospital, Peking University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2020.101227"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuros\/nyw078"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000146050.57722.2a"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12704"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.11.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2425"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192556"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.03.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.4836"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041415"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035665"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000146499.97948.52"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000000865"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-018-0731-y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8586-8596.1998"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1192753"},{"key":"e_1_2_8_18_1","article-title":"Hypoxia helps maintain nucleus pulposus homeostasis by balancing autophagy and apoptosis","volume":"13","author":"Kim HJ","year":"2020","journal-title":"Oxid Med Cell Longev"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(89)90288-8"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/13.2.105"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8TB02566E"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7068"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21197421"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.01.005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2017.08.004"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4867"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30154"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.18"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ17-0445"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3749"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2665"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2017.10.007"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.10.166"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(08)01416-X"}],"container-title":["JOR SPINE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsp2.1181","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsp2.1181","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsp2.1181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T04:25:01Z","timestamp":1693196701000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jsp2.1181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jsp2.1181"],"URL":"https:\/\/doi.org\/10.1002\/jsp2.1181","archive":["Portico"],"relation":{},"ISSN":["2572-1143","2572-1143"],"issn-type":[{"value":"2572-1143","type":"print"},{"value":"2572-1143","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"2021-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1181"}}