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Degeneration of the intervertebral disc due to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) allows nerves from dorsal root ganglia to grow into the core of the disc. Treatment with a decellularized tissue hydrogel that contains sGAGs may inhibit nerve growth and prevent disc\u2010associated LBP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A protocol to decellularize porcine nucleus pulposus (NP) was adapted from previous methods. DNA, sGAG, \u03b1\u2010gal antigen, and collagen content were analyzed before and after decellularization. The decellularized tissue was then enzymatically modified to be injectable and form a gel at 37\u00b0C. Following this, the mechanical properties, microstructure, cytotoxicity, and neuroinhibitory properties were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The decellularization process removed 99% of DNA and maintained 74% of sGAGs and 154% of collagen compared to the controls NPs. Rheology demonstrated that regelled NP exhibited properties similar to but slightly lower than collagen\u2010matched controls. Culture of NP cells in the regelled NP demonstrated an increase in metabolic activity and DNA content over 7\u2009days. The collagen content of the regelled NP stayed relatively constant over 7\u2009days. Analysis of the neuroinhibitory properties demonstrated regelled NP significantly inhibited neuronal growth compared to collagen controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The decellularization process developed here for porcine NP tissue was able to remove the antigenic material while maintaining the sGAG and collagen. This decellularized tissue was then able to be modified into a thermally forming gel that maintained the viability of cells and demonstrated robust neuroinhibitory properties in vitro. This biomaterial holds promise as an NP supplement to prevent nerve growth into the native disc and NP in vivo.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsp2.1187","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T08:45:21Z","timestamp":1641545121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Injectable decellularized nucleus pulposus tissue exhibits neuroinhibitory properties"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2428-7934","authenticated-orcid":false,"given":"Logan M.","family":"Piening","sequence":"first","affiliation":[{"name":"Biological Systems Engineering Department University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Lillyman","sequence":"additional","affiliation":[{"name":"Biological Systems Engineering Department University of 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