{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:36:21Z","timestamp":1780677381575,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005024","name":"Beijing Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023\u2010zz\u2010024"],"award-info":[{"award-number":["2023\u2010zz\u2010024"]}],"id":[{"id":"10.13039\/501100005024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JOR Spine"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Low back pain (LBP) is the main factor of global disease burden. Intervertebral disc degeneration (IVDD) has long been known as the leading reason of LBP. Increasing studies have verified that circular RNAs (circRNAs)\u2010microRNAs (miRNAs)\u2010mRNAs network is widely involved in the pathological processes of IVDD. However, no study was made to demonstrate the circRNAs\u2010mediated ferroptosis, oxidative stress, extracellular matrix metabolism, and immune response in IVDD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We collected 3 normal and 3 degenerative nucleus pulposus tissues to conduct RNA\u2010sequencing to identify the key circRNAs and miRNAs in IVDD. Bioinformatics analysis was then conducted to construct circRNAs\u2010miRNAs\u2010mRNAs interaction network associated with ferroptosis, oxidative stress, extracellular matrix metabolism, and immune response. We also performed animal experiments to validate the therapeutic effects of key circRNAs in IVDD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We found that circ_0015435 was most obviously upregulated and circ_0071922 was most obviously downregulated in IVDD using RNA\u2010sequencing. Then we observed that hsa\u2010miR\u201015a\u20105p was the key downstream of circ_0071922, and hsa\u2010miR\u201015a\u20105p was the top upregulated miRNA in IVDD. Bioinformatics analysis was conducted to predict that 56 immunity\u2010related genes, 29 ferroptosis\u2010related genes, 23 oxidative stress\u2010related genes and 8 ECM\u2010related genes are the targets mRNAs of hsa\u2010miR\u201015a\u20105p. Then we constructed a ceRNA network encompassing 24 circRNAs, 6 miRNAs, and 101 mRNAs. Additionally, we demonstrated that overexpression of circ_0071922 can alleviate IVDD progression in a rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The findings of this study suggested that circ_0071922\u2010miR\u201015a\u20105p\u2010mRNA signaling network might affect IVDD by modulating the nucleus pulposus cells ferroptosis, oxidative stress, ECM metabolism, and immune response, which is an effective therapeutic targets of IVDD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsp2.1275","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T06:33:33Z","timestamp":1692858813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Construction of circ_0071922\u2010miR\u201015a\u20105p\u2010mRNA network in intervertebral disc degeneration by 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