{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T12:45:18Z","timestamp":1782132318251,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:00:00Z","timestamp":1695513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:00:00Z","timestamp":1695513600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972098"],"award-info":[{"award-number":["81972098"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072490"],"award-info":[{"award-number":["82072490"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JOR Spine"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>An experimental study was performed to improve the anterior approach model of intervertebral disc degeneration (IVDD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The aims of this study were to investigate the anterior approach model of IVDD for the cause of death, phenotypes, and underlying mechanisms of low back pain in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      In this study, we conducted an anterior puncture procedure on a cohort of 300 C57BL\/6J mice that were 8\u2009weeks old. Our investigation focused on exploring the causes of death in the study population (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009300) and assessing the time\u2010course changes in various parameters, including radiographical, histological, immunofluorescence, and immunohistochemistry analyses (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910). Additionally, we conducted behavioral assessments on a subset of the animals (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200930).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Transverse vertebral artery rupture is a major factor in surgical death. Radiographical analyses showed that the hydration of the nucleus pulposus began to decrease at 2\u2009weeks after puncture and obviously disappeared over 4\u2009weeks. 3D\u2010CT showed that disc height was significantly decreased at 4\u2009weeks. Osteophyte at the anterior vertebral rims was observed at 2\u2009weeks after the puncture. As the time course increased, histological analyses showed progressive disruption of the destruction of the extracellular matrix and increased secretion of inflammatory cytokines and apoptosis. Behavioral signs of low back pain were increased between the puncture and sham groups at 4\u2009weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The improvement of anterior intervertebral disc approach model in mice will be useful to investigate underlying mechanisms and potential therapeutic strategies for behavior and phenotypes. Furthermore, the application of vibrational pre\u2010treatment can be used to increase the sensitivity of axial back pain in the model, thereby providing researchers with a reliable method for measuring this critical phenotype.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsp2.1284","type":"journal-article","created":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T22:20:31Z","timestamp":1695594031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Pain behavior and phenotype in a modified anterior lumbar disc puncture mouse model"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7693-6417","authenticated-orcid":false,"given":"Yuming","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Orthopaedics and Traumatology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linchuan","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Laboratory of General Surgery, The First Affiliated Hospital Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Orthopaedics and Traumatology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Orthopaedics and Traumatology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zemin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Orthopaedics and Traumatology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Orthopaedics and Traumatology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9747-0652","authenticated-orcid":false,"given":"Jianru","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1624-4136","authenticated-orcid":false,"given":"Zhaomin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"},{"name":"Sun Yan Sen University Pain Research Center  Guangzhou China"},{"name":"Guangdong Province Key Laboratory of Orthopaedics and Traumatology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp2032396"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-018-0154-x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1533-2500.2007.00171.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20669"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00165-x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2017.8"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000002427"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001284"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-19487-9"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.63.227"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.10.022"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100806"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e31816b46ca"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(87)90041-8"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.117"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2877-5"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.12.029"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e3181da38d5"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.747067"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203110"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60610-7"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26280"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-018-0125-y"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-015-0284-x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuros\/nyw078"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031715"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.12.025"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.0c04713"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2013.160"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/34214"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2020.101227"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.942435"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13162"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2019.02.004"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3823"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2012.01.027"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2015.08.055"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874325001105010072"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-199601010-00018"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00493"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.09.020"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02221600"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.135.4.7925134"}],"container-title":["JOR SPINE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsp2.1284","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsp2.1284","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsp2.1284","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T11:10:33Z","timestamp":1771240233000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jsp2.1284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,24]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/jsp2.1284"],"URL":"https:\/\/doi.org\/10.1002\/jsp2.1284","archive":["Portico"],"relation":{},"ISSN":["2572-1143","2572-1143"],"issn-type":[{"value":"2572-1143","type":"print"},{"value":"2572-1143","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,24]]},"assertion":[{"value":"2023-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1284"}}