{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:54:48Z","timestamp":1789408488211,"version":"build-2803163510"},"reference-count":100,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT\u2010178111"],"award-info":[{"award-number":["PJT\u2010178111"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JOR Spine"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Extracellular vesicles (EVs) function as biomarkers and are crucial in cell communication and regulation, with therapeutic potential for intervertebral disc (IVD)\u2010related low back pain (LBP). EV cargo is often affected by tissue health, which may affect the therapeutic potential. There is currently limited knowledge of how the cargo of IVD cell\u2010derived EVs varies with tissue health and how differences in proteomic profile affect the predicted biological functions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Our study purified EVs from human IVD cell conditioned media by size\u2010exclusion chromatography. Nanoparticle tracking analysis was conducted to measure EV size and concentration. Transmission electron microscopy and Western blot were performed to examine EV structure and markers. Tandem mass tag\u2010mass spectrometry was conducted to determine protein cargo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Most EVs were exosomes and intermediate microvesicles with an increasing amount linked to disease progression. Of the proteins detected, 88.6% were shared across the non\u2010degenerate, mildly\u2010degenerate, and degenerate samples. GO and KEGG analyses revealed that cargo from the mildly\u2010degenerate samples was the most distinct, with the proteins in high abundance strongly associated with extracellular matrix (ECM) organization and structure. Shared proteins, highly expressed in the non\u2010degenerate and degenerate samples, showed strong associations with cell adhesion, ECM\u2013receptor interaction, and vesicle\u2010mediated transport, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings indicate that EVs from IVD cells from tissue with different degrees of degeneration share a majority of the cargo proteins. However, the level of expression differs with degeneration grade. Cargo from the mildly\u2010degenerate samples exhibits the most differences. A better understanding of changes in EV cargo in the degenerative process may provide novel information related to molecular mechanisms underlying IVD degeneration and suggest new potential treatment modalities for IVD\u2010related LBP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsp2.70007","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T23:49:52Z","timestamp":1730850592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The proteomic landscape of extracellular vesicles derived from human intervertebral disc cells"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6342-0633","authenticated-orcid":false,"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"Department of Surgery, Division of Orthopaedics McGill University  Montreal Quebec Canada"},{"name":"The McGill Scoliosis and Spine Group, McGill University 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