{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:58:33Z","timestamp":1753869513125,"version":"3.41.2"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T00:00:00Z","timestamp":1740268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["NIH\/NIAMS","R21 AR078386","AR071623"],"award-info":[{"award-number":["NIH\/NIAMS","R21 AR078386","AR071623"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016787","name":"Penn Center for Musculoskeletal Disorders","doi-asserted-by":"publisher","award":["P30AR069619"],"award-info":[{"award-number":["P30AR069619"]}],"id":[{"id":"10.13039\/100016787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JOR Spine"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Back pain after intervertebral disc (IVD) injury is a common clinical problem. Previous work examining early molecular changes post injury mainly used a candidate marker approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, gene expression in the injured and intact mouse tail IVDs was determined with a nonbiased whole transcriptome approach and related to subsequent pain behavior. Mouse tail IVD injury was induced by a needle puncture. Whole murine transcriptome was determined by RNASeq. Transcriptomes of injured IVDs were compared with those of intact controls by bioinformatic methods. Mechanical allodynia was assessed by the Von Frey method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 17,722 murine genes with meaningful expressions, 7242 genes were differentially expressed (<jats:italic>P.adj<\/jats:italic> \u27e8\u20090.01). Ontology study of upregulated genes revealed that leukocyte migration was the most enriched biological process, and network analysis showed that Tnfa had the most protein\u2013protein interactions. The most enriched downregulated pathways were related to the pattern specification process. Mechanical allodynia persisted at the 4\u2010week end point.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The RNASeq data revealed numerous early genes that participate in inflammation and repair processes post IVD injury. Mechanical allodynia followed these gene expression changes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsp2.70049","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:25:39Z","timestamp":1741742739000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gene Expression Changes Precede Elevated Mechanical Sensitivity in the Mouse Intervertebral Disc Injury Model"],"prefix":"10.1002","volume":"8","author":[{"given":"Zuozhen","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Physical Medicine &amp; Rehabilitation Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Xiangya Hospital, Central South University  Changsha Hunan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frances S.","family":"Shofer","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brianna","family":"Ciesielski","sequence":"additional","affiliation":[{"name":"Neurobehavior Testing Core, Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Department of Orthopedic Surgery Tongji Hospital, Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Timothy","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Neurobehavior Testing Core, Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7484-8800","authenticated-orcid":false,"given":"Yejia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine &amp; Rehabilitation Perelman School of Medicine, University of Pennsylvania  Philadelphia Pennsylvania USA"},{"name":"Section of Rehabilitation Medicine, Corporal Michael J. 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