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The etiology of IDD is still unclear, but it has been largely demonstrated the crucial role of inflammation and neuroinflammation in the pathological and degenerative cascade of events characterizing IVD degeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>In this study, we evaluated the potential therapeutic effect of pulsed electromagnetic field (PEMF) on human degenerated IVD (D\u2010IVD) cells collected from patients who underwent discectomy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials &amp; Methods<\/jats:title><jats:p>The experimental plan to test our hypothesis, involved viability assay, reactive oxide species\/nitrite production, gene, and protein expression. To recapitulate the pro\u2010inflammatory disc microenvironment occurring during IDD, interleukin\u20101\u03b2 (IL\u20101\u03b2) was administered to IVD cell culture. Then, to dissect the contribution of neuroinflammatory condition to immune component, microglial cells were co\u2010cultured with IVD\u2010conditioned media, and viability and expression of inflammatory markers were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our data prove that in the IVD degenerative microenvironment, the increase of pro\u2010inflammatory mediators, extracellular matrix degradative enzymes, and neuroinflammatory markers could be reduced by PEMF therapy, resulting in an overall improvement of degenerative condition and LBP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results represent an impactful novelty for the management of people suffering from LPB, in terms of symptom relief and reduction of social\u2010health system burden.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsp2.70077","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T11:23:40Z","timestamp":1747653820000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In Vitro Validation of Pulsed Electromagnetic Field (<scp>PEMF<\/scp>) as an Effective Countermeasure Against Inflammatory\u2010Mediated Intervertebral Disc Degeneration"],"prefix":"10.1002","volume":"8","author":[{"given":"Laura","family":"Guarnaccia","sequence":"first","affiliation":[{"name":"Center of Aerospace Medicine for Advanced Therapies (CeMATA), Laboratory of Experimental Neurosurgery and Cell Therapy, Neurosurgery Unit Foundation IRCCS ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Begani","sequence":"additional","affiliation":[{"name":"Center of Aerospace Medicine for Advanced Therapies (CeMATA), Laboratory of Experimental Neurosurgery and Cell Therapy, Neurosurgery Unit Foundation IRCCS ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Pileggi","sequence":"additional","affiliation":[{"name":"Andremacon Srl  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Pluderi","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Borsa","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Fanizzi","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimiliano Domenico","family":"Rizzaro","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Fiore","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Fontana","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"},{"name":"Medical Genetics Unit ASST Santi Paolo e Carlo  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolando","family":"Campanella","sequence":"additional","affiliation":[{"name":"Andremacon Srl  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Cordiglieri","sequence":"additional","affiliation":[{"name":"Istituto Nazionale Genetica Molecolare Romeo Ed Enrica Invernizzi  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Gaudino","sequence":"additional","affiliation":[{"name":"Department of Neuroradiology Azienda Ospedaliero\u2010Universitaria Policlinico Umberto I  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni A.","family":"Alotta","sequence":"additional","affiliation":[{"name":"Andremacon Srl  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Miozzo","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan Italy"},{"name":"Medical Genetics Unit ASST Santi Paolo e Carlo  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Garzia","sequence":"additional","affiliation":[{"name":"Aerospace Medicine Institute \u201cA. 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