{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:14:53Z","timestamp":1790190893180,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["tstp20250511"],"award-info":[{"award-number":["tstp20250511"]}],"id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JOR Spine"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Adolescent idiopathic scoliosis (AIS) is linked to mechanical stress\u2010induced growth plate dysfunction. PIEZO1 (mechanosensitive ion channel) mediates chondrocyte apoptosis via calcium, and primary cilia (mechanosensory organelles) regulate cartilage matrix synthesis, but their interplay in stress\u2010induced growth plate degeneration\/ossification remains unclear; this study explored the PIEZO1\u2010primary cilia axis's role.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Conditional IFT88 knockout (IFT88\u2010cKO) mice were generated via\n                      <jats:italic>Col2a1<\/jats:italic>\n                      \u2010\n                      <jats:italic>CreERT<\/jats:italic>\n                      and\n                      <jats:italic>Ift88<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>flox \/<\/jats:italic>\n                      <\/jats:sup>\n                      <jats:sup>\n                        <jats:italic>flox<\/jats:italic>\n                      <\/jats:sup>\n                      crosses (tamoxifen\u2010induced). 8\u2010week\u2010old C57BL\/6J mice were divided into control, wild\u2010type caudal compression (CC, 10\u2009kPa), and IFT88\u2010CKO CC groups (samples at 8\u2009weeks, MRI at 4\u2009weeks). Primary growth plate chondrocytes from 5\u2010day\u2010old C57BL\/6J mice were cultured, stressed (self\u2010developed device, 100\u2009kPa), or treated with Yoda1 (PIEZO1 agonist)\/chloral hydrate (cilia disruptor)\/siRNA (PIEZO1\/IFT88 knockdown). Detection included Western blot, RT\u2010qPCR, immunofluorescence, immunohistochemistry, ARS\/ALP\/ABS\/HE\/Safranine O staining, and transcriptome sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PIEZO1 co\u2010localized with primary cilia (ac\u2010\u03b1\u2010Tubulin labeled) in chondrocytes; chloral hydrate reduced cil\u2010ia\u2010positive cells (97.2% \u2192\u200916.6%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Stress upregulated PIEZO1, decreased COL2A1 (cartilage marker), increased RUNX2 (osteogenic marker), and enhanced ALP\/ARS staining. Transcriptome identified 11,534 differ\u2010entially expressed genes (e.g., upregulated PIEZO1\/IFT88, downregulated ACAN). SiPIEZO1 reduced IFT88 (non\u2010stress), while siIFT88 feedback\u2010upregulated PIEZO1 (stress). Yoda1 induced cartilage degeneration, which was reversed by siIFT88\/chloral hydrate. In\u00a0vivo, CC increased disc degeneration score (2 \u2192\u200912), while IFT88\u2010cKO reduced it to 8 and attenuated COL2A1 downregulation\/RUNX2 upregulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The PIEZO1\u2010primary cilia axis mediates stress\u2010induced growth plate degeneration\/ossification: stress upregulates PIEZO1, which relies on primary cilia to promote chondrocyte apoptosis and abnormal ossification; cilia disruption alleviates these effects. This provides insights into AIS pathogenesis and identifies PIEZO1\/primary cilia as therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsp2.70133","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T01:51:43Z","timestamp":1762307503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>PIEZO1<\/scp>\n                    \u2010Primary Cilia Axis Mediates Compressive Stress\u2010Induced Growth Plate Degeneration and Ossification in Adolescent Idiopathic Scoliosis"],"prefix":"10.1002","volume":"8","author":[{"given":"Fei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fushuai","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukun","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3520-2281","authenticated-orcid":false,"given":"Jianyi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Shandong Public Health Clinical Center Shandong University  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7529-6542","authenticated-orcid":false,"given":"Zichen","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingzhi","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9165-3239","authenticated-orcid":false,"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2921-0699","authenticated-orcid":false,"given":"Yongming","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery The Affiliated Hospital of Qingdao University  Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"issue":"4","key":"e_1_2_12_2_1","first-page":"803","article-title":"Longitudinal Growth of the Human Vertebra; a Contribution to Human Osteogeny","volume":"32","author":"Bick E. 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