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(b) This site fractionates into an enzyme complex (complex V), which is capable of oligomycin\u2010 and uncoupler\u2010sensitive ATP\u2010Pi exchange. It is absent from electron transfer complexes I, III, and IV, which represent segments of the respiratory chain containing coupling sites 1, 2, and 3, respectively. (c) Trinitrophenol is a membrane\u2010impermeable uncoupler (uncouples submitochondrial particles, but not mitochondria) and a poor protonophore. There is an excellent correlation between the uncoupling potencies and the affinities of uncouplers for the mitochondrial uncoupler\u2010binding site. There is no correlation between uncoupling potency and protonophoric activity of uncouplers when a membrane\u2010permeable uncoupler is compared with a membrane\u2010impermeable one.<\/jats:p>","DOI":"10.1002\/jss.400030302","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:49:29Z","timestamp":1117273769000},"page":"201-213","source":"Crossref","is-referenced-by-count":27,"title":["Energy conservation and uncoupling in mitochondria"],"prefix":"10.1002","volume":"3","author":[{"given":"Youssef","family":"Hatefi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"327","volume-title":"Comprehensive Biochemistry","author":"Slater E. 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