{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T12:12:20Z","timestamp":1693483940572},"reference-count":38,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":10641,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Supramol Struct"],"published-print":{"date-parts":[[1975,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epithelial cells (80\u201390% \u201cgranular\u201d type) were isolated from urinary bladders of <jats:italic>Bufo marinus<\/jats:italic> and <jats:italic>Rana catesbiana<\/jats:italic>. The inhibitory effect of \u03b1\u2010methyl\u2010D\u2010mannoside on fluorescein\u2010labeled concanavalin A (Con A) binding to these cells indicates that they possess specific binding sites for Con A. The lectin also mediates adsorption of erythrocytes to these cells. Both Con A binding and Con A\u2010mediated hem\u2010adsorption to epithelial cells are depressed at 4\u00b0C, as compared with cells maintained at 22\u00b0C. Elevation of temperature to 37\u00b0C, however, enhances hemadsorption independently of alterations in lectin binding. Treatment of cells with antidiuretic hormone (ADH) at 22\u00b0C followed by 15 min of incubation at 22\u00b0 or 37\u00b0C before exposure of cells to Con A promotes increments in Con A\u2010mediated hemadsorption, but not in lectin binding, at 22\u00b0 or 37\u00b0C. These hormonal effects are not significant when hemadsorption is assayed at 4\u00b0C. Treatment of cells with another octapeptide, angiotensin, elicits a small, but significant, increment in hemadsorption to epithelial cells which is likewise uninfluenced by quantitative changes in lectin binding. Collectively, these data and other independent observations suggest that treatment with octapeptide hormones acts to enhance the redistribution and aggregation of lectin\u2010binding proteins in the membranes of granular epithelial cells from amphibian urinary bladder. Such changes, in turn, may contribute to the alterations in membrane transport properties which characterize the hormonal response.<\/jats:p>","DOI":"10.1002\/jss.400030502","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T09:50:49Z","timestamp":1117273849000},"page":"391-400","source":"Crossref","is-referenced-by-count":5,"title":["Surface modifications evoked by antidiuretic hormone in isolated epithelial cells: Evidence from lectin probes"],"prefix":"10.1002","volume":"3","author":[{"given":"Richard J.","family":"Pietras","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Naujokaitis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara M.","family":"Szego","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.45.5.921"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(72)90110-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/247222a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(70)90625-X"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4023.720"},{"key":"e_1_2_1_7_2","first-page":"91","volume":"32","author":"Burger M. 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