{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:19:45Z","timestamp":1762100385508},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":9180,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Supramol Struct"],"published-print":{"date-parts":[[1979,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present work examined the expression of cell surface glycoprotein antigens in cultured human cell lines. The set of glycoproteins studied was defined by their immunoreactivity with antiserum developed to Triton\u2010solubilized extracts of placental brush border membranes. Studies were performed using cell lines of trophoblastic (BeWo, JEG\u20103) and nontrophoblastic (Chang liver cells) origin, as well as diploid fibroblast cell lines (WI\u201038, GM\u201038).<\/jats:p><jats:p>Antiplacental brush border antiserum reacts with at least 19 distinct antigens present in placental membrane preparations, each of which can be resolved and identified in two\u2010dimensional electrophoresis. The subunit molecular weight and isoelectric point for all components were defined by their positions in the two\u2010dimensional matrix. Thirteen of these could be detected among the five cell lines examined by lactoperoxidase\u2010catalyzed cell surface iodination.<\/jats:p><jats:p> One of these 13 antigens has been identified as the placental isoenzyme of alkaline phosphatase (PAP). The expression of this component is limited to choriocarcinonia cells and Chang liver cells and it is not present in diploid fibroblasts. Under normal circumstances expression of PAP is unique to the differentiated placenta but has been frequently demonstrated in both trophoblastic and nontrophoblastic neoplasms.<\/jats:p><jats:p>Two other antigens are variably expressed among the different cell types examined in the present study and their presence or absence was independent of the trophoblastic, epithelial nontrophoblastic, or fibroblastic origin of the cells.<\/jats:p><jats:p>Ten surface antigens were expressed in all five cell lines. Six of these had previously been found common to membranes from three adult differentiated tissues, including liver and kidney, as well as placenta (Wada et al, J Supramol Struc 10(3):287\u2013305, 1979). The presence of this set of antigens in cultured cells as well extends the possibility that these are ubiquitously expressed on human cell surfaces. Two other antigens observed in all cultured cells had been found in both placental and either kidney or liver membranes and may represent common functions shared by many tissues which are also necessary for growth in vitro. The two remaining placental antigens seen in all cultured cells have previously been shown to be absent in adult tissues. Their presence in cultured cells but not in the membranes of resting differentiated tissues may signify the expression of glycoproteins characteristic of trophoblasts in all cells adapted to growth in culture.<\/jats:p>","DOI":"10.1002\/jss.400110409","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T20:07:08Z","timestamp":1117224428000},"page":"503-515","source":"Crossref","is-referenced-by-count":12,"title":["Human placental cell surface antigens: Expression by cultured cells of diverse phenotypic origin"],"prefix":"10.1002","volume":"11","author":[{"given":"Thomas A.","family":"Hamilton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Garrett","family":"Wada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard H.","family":"Sussman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"27","volume-title":"Advances in Reproductive Physiology","author":"Billington WD","year":"1971"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0474-36"},{"key":"e_1_2_1_4_2","first-page":"1","volume":"458","author":"Nicolson GL","year":"1976","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_1_5_2","first-page":"709","volume-title":"Biological Interactions in Normal and Neoplastic Growth","author":"Gasic G","year":"1961"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.169.3945.601"},{"key":"e_1_2_1_7_2","unstructured":"SussmanHH: In Ruoslahti E Engvall E (eds): \u201cChemistry of Tumor\u2010Associated Antigens.\u201dScand J Immunol(Suppl 6) 1978 pp126\u2013140."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.4.1947"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400060402"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400100303"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-102-25149"},{"key":"e_1_2_1_12_2","first-page":"1231","volume":"28","author":"Pattillo RH","year":"1968","journal-title":"Cancer Res"},{"key":"e_1_2_1_13_2","first-page":"45","volume-title":"Embryonic and Fetal Antigens in Cancer","author":"Pattillo RH","year":"1972"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-32-5-683"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-87-21406"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.1.323"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040910112"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.115.6.1617"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43072-4"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99338-5"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(73)90217-0"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_23_2","unstructured":"HamiltonTA WadaHG SussmanHH:Scand J Imm(in press)."},{"key":"e_1_2_1_24_2","first-page":"93","volume":"6","author":"Williams AF","year":"1977","journal-title":"Contemporary Topics in Molecular Immunology"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90296-9"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61793-4"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61794-6"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1159\/000130357"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01542970"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4453"}],"container-title":["Journal of Supramolecular Structure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjss.400110409","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jss.400110409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T14:35:22Z","timestamp":1698849322000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jss.400110409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,1]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1979,1]]}},"alternative-id":["10.1002\/jss.400110409"],"URL":"https:\/\/doi.org\/10.1002\/jss.400110409","archive":["Portico"],"relation":{},"ISSN":["0091-7419","1547-9366"],"issn-type":[{"value":"0091-7419","type":"print"},{"value":"1547-9366","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,1]]}}}