{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:36:44Z","timestamp":1787236604890,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,3,8]],"date-time":"2004-03-08T00:00:00Z","timestamp":1078704000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A sensitive method for determination of disaturated phosphatidylserine species in the presence of their monounsaturated analogs has been developed, using micro liquid chromatography coupled to electrospray ionization tandem mass spectrometry. The hydrophobic nature of the phosphatidylserine species required a combination of low\u2010eluting sample solvents and sub\u2010ambient temperatures in order to focus large sample volumes up to 20 \u03bcL. The samples were dissolved in 2\u2010propanol\u00a0:\u00a0hexane\u00a0:\u00a0water (20\u00a0:\u00a010\u00a0:\u00a04,\n                    <jats:italic>v\/v\/v<\/jats:italic>\n                    ) prior to 1\u00a0:\u00a09 dilution with ammonium formate buffer\u00a0:\u00a02\u2010propanol\u00a0:\u00a0tetrahydrofuran (30\u00a0:\u00a055\u00a0:\u00a015,\n                    <jats:italic>v\/v\/v<\/jats:italic>\n                    ) and final 1\u00a0:\u00a04 dilution with ammonium formate buffer (10 mM)\u00a0:\u00a02\u2010propanol\u00a0:\u00a0tetrahydrofuran (55\u00a0:\u00a037.5\u00a0:\u00a07.5,\n                    <jats:italic>v\/v\/v<\/jats:italic>\n                    ). The analytical column was a 0.5\u00d7150 mm stainless steel column packed with 5 \u03bcm C\n                    <jats:sub>30<\/jats:sub>\n                    particles, while the mobile phase contained ammonium formate buffer (10 mM)\u00a0:\u00a0 2\u2010propanol\u00a0:\u00a0tetrahydrofuran (30\u00a0:\u00a055\u00a0:\u00a015,\n                    <jats:italic>v\/v\/v<\/jats:italic>\n                    ). A temperature program from 5\u00b0C (hold for 3 minutes) to 75\u00b0C at 8 K\/min provided separation of the disaturated phosphatidylserine species from their monounsaturated analogs, making available a sensitive determination of the isobaric species. The mass limit of detection for dipalmitoyl phosphatidylserine was 100 pg, corresponding to a concentration limit of detection of 5 pg\/\u03bcL when using an injection volume of 20 \u03bcL. This is an improvement by a factor of 20 as compared to previously reported numbers obtained with conventional LC columns. The within\u2010assay precision of dipalmitoyl phosphatidylserine was 11.9% RSD (\n                    <jats:italic>n<\/jats:italic>\n                    = 3), while the retention time precision was 4.1% RSD (\n                    <jats:italic>n<\/jats:italic>\n                    = 6).\n                  <\/jats:p>","DOI":"10.1002\/jssc.200301706","type":"journal-article","created":{"date-parts":[[2004,3,9]],"date-time":"2004-03-09T11:02:49Z","timestamp":1078830169000},"page":"297-303","source":"Crossref","is-referenced-by-count":13,"title":["Temperature optimization for improved determination of phosphatidylserine species by micro liquid chromatography with electrospray tandem mass spectrometric detection"],"prefix":"10.1002","volume":"27","author":[{"given":"\u00c5smund","family":"Larsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paal","family":"Molander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,3,8]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"B.Alberts D.Bray J.Lewis M.Raft K.Roberts J.D.Watson in:Molecular biology of the cell 3rd ed. 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