{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:38:40Z","timestamp":1791725920991,"version":"4.3.4"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,7,29]],"date-time":"2004-07-29T00:00:00Z","timestamp":1091059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ability of reversed\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) to separate some positionally isomeric disaturated and monounsaturated triacylglycerols (TAGs) as intact species is demonstrated for the first time. Mobile phases of acetonitrile modified with methanol, ethanol, 2\u2010propanol, 1\u2010propanol, 1\u2010butanol, acetone, or dichloromethane were tested for the separation of POP\u2010PPO, PLP\u2010PPL, PEP\u2010PPE, and PDP\u2010PPD (P\u00a0\u2013\u00a0palmitic, O\u00a0\u2013\u00a0oleic, L\u00a0\u2013\u00a0linoleic, E\u00a0\u2013\u00a0eicosapentaenoic, D\u00a0\u2013\u00a0docosahexaenoic acid residue) on a single RP\u2010HPLC column. The resolution improved with increasing number of double bonds in the acyl residues. While POP and PPO were only partially resolved, PDP and PPD were fully separated with all tested mobile phases, except those containing methanol. Also separated were the four TAGs having the same equivalent carbon number (ECN = 42), PEP, PPE, PDP, and PPD, on a single RP\u2010HPLC column with mobile phase acetonitrile<jats:italic>\u2013<\/jats:italic>2\u2010propanol (70:30, <jats:italic>v\/v<\/jats:italic>) at 0.8 mL\/min. In all cases the isomer with the unsaturated acyl residue in either 1\u2010 or 3\u2010position was retained more strongly than the respective 2\u2010isomer.<\/jats:p>","DOI":"10.1002\/jssc.200401746","type":"journal-article","created":{"date-parts":[[2004,7,29]],"date-time":"2004-07-29T03:01:40Z","timestamp":1091070100000},"page":"1033-1036","source":"Crossref","is-referenced-by-count":51,"title":["Resolution of triacylglycerol positional isomers by reversed\u2010phase high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"27","author":[{"given":"Svetlana","family":"Momchilova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichiro","family":"Tsuji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Itabashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boryana","family":"Nikolova\u2010Damyanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnis","family":"Kuksis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(02)74079-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1193"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/77.4.777"},{"key":"e_1_2_1_5_2","unstructured":"A.Kuksis in:Advances in Lipid Methodology \u2013 Three W.W. 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