{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:41:55Z","timestamp":1760920915211,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,7,12]],"date-time":"2004-07-12T00:00:00Z","timestamp":1089590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of the current study was to characterize <jats:italic>Leishmania<\/jats:italic> cell\u2010surface antigens by two different methods established for the purification of glycoproteins and proteins, and to point out a useful approach to define their size and mass heterogeneity. <jats:italic>L. tropica <\/jats:italic>parasites were initially isolated from patients with active cutaneous leishmaniasis and were then cultured in vitro. The parasite\u2010cell layer was solubilised with 6 M guanidinium chloride (GuHCl) and subsequently prepared for the purification procedure. The methods used in this work were gel filtration chromatography and isopycnic density\u2010gradient centrifugation. Because of the presence of a substantial amount of non\u2010specific proteins in the culture medium, these methods were not effective alone in distinguishing these antigens. However, a good idea of their N\u2010glycosylated structures could be obtained by using Periodic acid\u2010Schiffs (PAS) and Con A lectin, and also size and mass heterogeneity. A combination of these methods effected a clear separation of the antigens. Amino acid analysis of the purified antigens was performed to positively identify them as well\u2010known <jats:italic>Leishmania<\/jats:italic> cell\u2010surface antigen gene products. The results confirmed the presence of more than one cell\u2010surface antigen on the <jats:italic>Leishmania <\/jats:italic>parasite and the combination of gel chromatography and density\u2010gradient centrifugation could be useful for their isolation.<\/jats:p>","DOI":"10.1002\/jssc.200401747","type":"journal-article","created":{"date-parts":[[2004,7,12]],"date-time":"2004-07-12T05:55:22Z","timestamp":1089611722000},"page":"1011-1016","source":"Crossref","is-referenced-by-count":1,"title":["A preliminary approach to the separation of <i>Leishmania <\/i>cell\u2010surface antigens"],"prefix":"10.1002","volume":"27","author":[{"given":"Nurten","family":"Aksoy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatice","family":"Ozbilge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sait","family":"Keles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet","family":"Iriadam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00fcseyin","family":"Vural","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatih","family":"Akcay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(86)90149-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"742","DOI":"10.4049\/jimmunol.146.2.742","volume":"146","author":"Burns J.M.","year":"1991","journal-title":"J. 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