{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:40:36Z","timestamp":1762864836920,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"17-18","license":[{"start":{"date-parts":[[2004,11,15]],"date-time":"2004-11-15T00:00:00Z","timestamp":1100476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report the development of efficient electrophoretic methods for the separation and quantification of L\u2010arginine and six naturally occurring derivatives that are structurally and functionally related. Capillary electrophoresis (CE) employing a concentrated borate buffer at pH 9.4 achieves the separation of mixtures containing dimethyl\u2010L\u2010arginine, <jats:italic>N<\/jats:italic><jats:sup>\u00a0G<\/jats:sup>\u2010monomethyl\u2010L\u2010arginine, L\u2010arginine, L\u2010homoarginine, L\u2010ornithine, and L\u2010citrulline as 4\u2010fluoro\u20107\u2010nitrobenzofurazan derivatives. In addition, the separation of the isomeric dimethyl\u2010L\u2010arginine derivatives (symmetric and asymmetric) is attained with baseline resolution by micellar electrokinetic chromatography (MEKC) when a high concentration of deoxycholic acid is added as a surfactant to the same running buffer. The influence of buffer type, concentration, and pH on the separation was studied to optimize separation conditions. The limit of quantitation (LOQ) for asymmetric dimethyl\u2010L\u2010arginine in aqueous solution was determined to be 20 \u03bcM using UV absorption in a CE separation and 0.1 \u03bcM using laser induced fluorescence (LIF) detection in an MEKC separation. This newly developed method was successfully applied for the quantitation of asymmetric dimethyl\u2010L\u2010arginine and L\u2010arginine in human plasma samples at levels that might be used as a clinical diagnostic for cardiovascular disease (0.125 \u03bcM LOQ).<\/jats:p>","DOI":"10.1002\/jssc.200401918","type":"journal-article","created":{"date-parts":[[2004,11,15]],"date-time":"2004-11-15T09:09:32Z","timestamp":1100509772000},"page":"1483-1490","source":"Crossref","is-referenced-by-count":31,"title":["Capillary electrophoretic and micellar electrokinetic separations of asymmetric dimethyl\u2010L\u2010arginine and structurally related amino acids: Quantitation in human plasma"],"prefix":"10.1002","volume":"27","author":[{"given":"Gabriele","family":"Trapp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"Sydow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria T.","family":"Dulay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Cooke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard N.","family":"Zare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19990712)38:13\/14<1882::AID-ANIE1882>3.0.CO;2-V"},{"key":"e_1_2_1_3_2","first-page":"572","author":"Vallance P.","year":"1992","journal-title":"Lancet"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.9.2032"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20000173"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)07217-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000126823.07732.D5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199912000-00016"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)94526-6"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00525-7"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9314(20020701)25:10\/11<665::AID-JSSC665>3.0.CO;2-H"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00034-7"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00399-6"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.09.050"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.09.001"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","unstructured":"R.Kuhn S.Hofstetter\u2010Kuhn Capillary Electrophoresis: Principles and Practice Springer Verlag Berlin 1993 p. 36.","DOI":"10.1007\/978-3-642-78058-5_2"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00632-8"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200401918","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.200401918","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:13:28Z","timestamp":1760872408000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.200401918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":16,"journal-issue":{"issue":"17-18","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1002\/jssc.200401918"],"URL":"https:\/\/doi.org\/10.1002\/jssc.200401918","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2004,12]]}}}