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Analysis was performed in less than 4.5 min in isocratic mode on a reversed\u2010phase C<jats:sub>18<\/jats:sub> column (5 \u03bcm; 150 \u00d7 4.6 mm) using a mobile phase composed of acetonitrile\u2010buffer phosphate 0.01 M (60:40, <jats:italic>v\/v<\/jats:italic>) adjusted to pH 6.0, at 1 mL\/min flow rate and UV absorbance at 210 nm. The elution order of analytes was dextromethorphan (DXM), Internal Standard (IS), and phenytoin (PHT). Calibration curves were linear in the 7.5\u201325 \u03bcg\/mL range for PHT and in the 10\u201330 \u03bcg\/mL range for DXM. Spike recoveries for urine samples prepared at three spiking levels ranged from 97.8 to 102.3% for PHT and from 94.8 to 100.4% for DXM. The detection limit (LOD) values ranged from 0.08 \u03bcg\/mL for PHT to 0.5 \u03bcg\/mL for DXM. The quantitation limit (LOQ) values ranged from 0.3 \u03bcg\/mL for PHT to 1.6 \u03bcg\/mL for DXM. The sample preparation method involves a rapid and simple procedure based on solid\u2010phase extraction using a C<jats:sub>18 <\/jats:sub>reversed\u2010phase column. Validation of the optimised method was carried out according to the ICH guidelines. The method developed in this study allows the reliable simultaneous analysis of PHT and DXM, drugs that were never quantified together in previously reported analytical methods. The described method has the advantage of being rapid and easy and it could be applied in therapeutic monitoring of these drugs in human urine of epileptic patients.<\/jats:p>","DOI":"10.1002\/jssc.200401942","type":"journal-article","created":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T03:21:57Z","timestamp":1119928917000},"page":"1157-1162","source":"Crossref","is-referenced-by-count":23,"title":["Simultaneous determination of phenytoin and dextromethorphan in urine by solid\u2010phase extraction and HPLC\u2013DAD"],"prefix":"10.1002","volume":"28","author":[{"given":"Natale Alfredo","family":"Santagati","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Gotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Ronsisvalle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,7,14]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"G.K.Lloyd G.Gillenwater Principles of Pharmacology: Basic Concept and Clinical Application; Epilepsy and Antiepileptic Drugs. 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