{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:38:45Z","timestamp":1791725925785,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,7,19]],"date-time":"2005-07-19T00:00:00Z","timestamp":1121731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The main constituents of plant oils are complex mixtures of TGs differing in acyl chain lengths, number and positions of double bonds, and regioisomerism. A non\u2010aqueous reversed\u2010phase HPLC method with acetonitrile\u20132\u2010propanol gradient and 30+15 cm NovaPak C<jats:sub>18<\/jats:sub> columns makes possible an unambiguous identification of the highest number of TGs ever reported for these oils, based on positive\u2010ion APCI mass spectra. A new approach to TG quantitation is based on the use of response factors with three typical detection techniques for that purpose (APCI\u2010MS, evaporative light\u2010scattering detection, and UV at 205 nm). Response factors of 23 single\u2010acid TGs (saturated TGs from C7 to C22, 7 unsaturated TGs), 4 mixed\u2010acid TGs, diolein and monoolein are calculated from their calibration curves and related to OOO. Due to differences between saturated and unsaturated acyl chains, the use of response factors significantly improves the quantitation of TGs. 133 TGs containing 22 fatty acids with 8\u201325 carbon atoms and 0\u20133 double bonds are identified and quantified in 9 plant oils (walnut, hazelnut, cashew nut, almond, poppy seed, yellow melon, mango, fig, date) using HPLC\/APCI\u2010MS with a response factor approach. Average parameters and relative fatty acid concentrations are calculated with both HPLC\/APCI\u2010MS and GC\/FID.<\/jats:p>","DOI":"10.1002\/jssc.200500088","type":"journal-article","created":{"date-parts":[[2005,7,19]],"date-time":"2005-07-19T10:20:05Z","timestamp":1121768405000},"page":"1315-1333","source":"Crossref","is-referenced-by-count":199,"title":["Quantitation of triacylglycerols in plant oils using HPLC with APCI\u2010MS, evaporative light\u2010scattering, and UV detection"],"prefix":"10.1002","volume":"28","author":[{"given":"Michal","family":"Hol\u010dapek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"L\u00edsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Jandera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nad\u011b\u017eda","family":"Kab\u00e1tov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.11.070191.002213"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/02648725.1999.10647973"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)01030-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(199708)11:12<1240::AID-RCM990>3.0.CO;2-5"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00861-9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-997-0060-x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200301610"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/20014091076686"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00790-6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)01233-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1193"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.04.063"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00352-6"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1317"},{"key":"e_1_2_1_16_2","first-page":"219","volume":"1","author":"H\u00e9ron S.","year":"1994","journal-title":"OCL\u2010Oleagineux Corps Gras Lipides"},{"key":"e_1_2_1_17_2","unstructured":"S.H\u00e9ron A.Tchapla Fingerprints of Triacyglycerols from Oils and Fats by HPLC Isocratic Elution and Evaporative Light Scattering Detection ELSD Sedex 45 Sedere Alfortville France 1994."},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02522685"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-120003275"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-100103414"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00553-6"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.581"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0725-5"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf010015w"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1601096"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-667X(1997)9:5<409::AID-MCS7>3.0.CO;2-5"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)88620-3"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00277-5"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02587918"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)00129-2"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)01311-0"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02543018"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac991312r"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.02.052"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200301538"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/jhrc.1240150404"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/pca.721"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(93)80458-K"},{"key":"e_1_2_1_39_2","doi-asserted-by":"crossref","unstructured":"S.Hamilton R.J.Hamilton P.A.Sewell: Extraction of lipids and derivative formation. 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