{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:45Z","timestamp":1786978125428,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T00:00:00Z","timestamp":1126483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A micellar electrokinetic chromatography method has been developed for simultaneous determination of melatonin and its precursors and metabolites. A 20 mM borate buffer pH 9.5 with 50 mM SDS served as the electrolyte. Tryptophan, 5\u2010methoxyindoleacetic acid, 6\u2010hydroxymelatonin, melatonin, serotonin, and 5\u2010methoxytryptamine were baseline separated in less than 13 min. The limits of detection for UV detection and fluorometric detection based on native fluorescence of analytes were at the sub\u2010ppm level. The proposed method with UV detection was applied to melatonin content control in pharmaceutical tablets with a precision expressed as RSD (\n                    <jats:italic>n<\/jats:italic>\n                    = 7) = 1.6%. For biological samples extraction with chloroform and ethyl acetate was examined. With ethyl acetate and chloroform recoveries of 87.2% and 82.1% melatonin, respectively, were obtained from plasma samples. The recovery of melatonin from spiked urine samples was 80.0% for ethyl acetate and 82.5% for chloroform. Fluorometric detection provides about two\u2010fold improvement over UV in the detection of melatonin and minor improvements for three other analytes, but is much poorer than UV for tryptophan and 6\u2010hydroxymelatonin in applied conditions.\n                  <\/jats:p>","DOI":"10.1002\/jssc.200500095","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T05:16:49Z","timestamp":1126502209000},"page":"2165-2172","source":"Crossref","is-referenced-by-count":31,"title":["Determination of melatonin and its precursors and metabolites using capillary electrophoresis with UV and fluorometric detection"],"prefix":"10.1002","volume":"28","author":[{"given":"Ewa","family":"Pobo\u017cy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksander","family":"Michalski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Sotowska\u2010Brochocka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Trojanowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,10,25]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Yu H. 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