{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:41:35Z","timestamp":1790862095169,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2005,10,24]],"date-time":"2005-10-24T00:00:00Z","timestamp":1130112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A high\u2010performance liquid chromatographic method with diode\u2010array detection, at 351 nm, was developed and validated for the determination of five tetracyclines (TCs): minocycline, tetracycline, oxytetracycline, chlortetracycline, and doxycycline in bovine muscle. Samples were macerated with a buffer solution, centrifuged, and purified using Abselut Nexus SPE cartridges. The separation of the examined TCs was achieved on an Inertsil ODS\u20103 5 \u03bcm, 250\u00d74 mm analytical column, at ambient temperature. A multistep gradient elution was followed using 0.05 M oxalic acid and CH<jats:sub>3<\/jats:sub>CN, at a flow rate of 1.65 mL\/min. The procedure was validated according to the European Union regulation 2002\/657\/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. The results of the validation process demonstrate that the method can be readily applied to European Union statutory veterinary drug residue surveillance programmes. Mean recoveries of TCs from bovine muscle samples spiked at three concentrations (100, 250, and 400 ng\/g) were in the range of 98.7\u2013103.3%. Method's LOQ values achieved were 40 \u03bcg\/kg for MNC, CTC, and DC and 25 \u03bcg\/kg for OTC and TC. The decision limits (CC\u03b1) were in the range of 104.7\u2013109.8 \u03bcg\/kg, while the detection capability (CC\u03b2) was in the range of 108.4\u2013116.7 \u03bcg\/kg for all compounds.<\/jats:p>","DOI":"10.1002\/jssc.200500160","type":"journal-article","created":{"date-parts":[[2005,10,24]],"date-time":"2005-10-24T05:48:10Z","timestamp":1130132890000},"page":"2247-2258","source":"Crossref","is-referenced-by-count":44,"title":["Development and validation of an HPLC confirma\u2010tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002\/657\/EC"],"prefix":"10.1002","volume":"28","author":[{"given":"Victoria F.","family":"Samanidou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantina I.","family":"Nikolaidou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis N.","family":"Papadoyannis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.17221\/5681-VETMED","volume":"49","author":"Michalova E.","year":"2004","journal-title":"Vet. 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Commun.1996 L125 10\u201332."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.02.037"},{"key":"e_1_2_1_7_2","unstructured":"2002\/657\/EC: Commission Decision of 12 August 2002 implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and the interpretation of results Off. J. Eur. 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