{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T04:59:44Z","timestamp":1768885184424,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,17]],"date-time":"2006-01-17T00:00:00Z","timestamp":1137456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Solvent extraction, sonication, and microwave\u2010assisted extractions in the presence of extraction agents (thioacetic acid, citric acid, cysteine, 2\u2010mercaptoethanol, HCl + NaCl,<jats:italic> etc.<\/jats:italic>) were tested for the isolation of mercury species. A mixture of 6 M HCl and 0.1 M NaCl was selected as the most suitable extraction agent. The extraction efficiency was about 10% higher and the RSD below 3.3% when microwave\u2010assisted extraction was applied instead of sonication. The liquid chromatography\u2013cold vapour atomic fluorescence spectrometry (LC\/CV\u2010AFS) method was optimised and used for separation and determination of inorganic mercury cations and alkylated and arylated mercury species. Isocratic elution at a flow rate of 0.15 mL\/min (with a mobile phase containing 0.05% 2\u2010mercaptoethanol (pH = 5) and 7% methanol and with a stepwise increase of methanol content up to 100% MeOH in the 15th min) was used for separation of mercury species on a Hypersil BDS C18 RP column. The limits of detection of the LC\/CV\u2010AFS system were estimated as 0.2 \u03bcg\/L (3%) for MeHg<jats:sup>+<\/jats:sup>, 0.07 \u03bcg\/L (5.3%) for inorganic Hg, 0.06 \u03bcg\/L (3.4%) for PhHg<jats:sup>+<\/jats:sup>, and 0.12 \u03bcg\/L (4.4%) for EtHg with the corresponding RSDs at 5 \u03bcg\/L (<jats:italic>n <\/jats:italic>= 10) given in parentheses. The concentrations (2\u201310 mg\/kg fresh weight) of total mercury and methylmercury (90\u201399% of the total mercury) in selected fish obtained by HPLC\/CV\u2010AFS were in good agreement (absolute deviations 0.05 mg\/kg) but more precise (RSDs &lt;5.4% at 5 mg\/L,<jats:italic> n<\/jats:italic> = 10) than those determined by GC coupled to an electron capture detector. The RSDs (3.1\u20138.2% and 4.1\u20139.0%) of the overall analytical procedure for the determination of total mercury (AMA 254) and methylmercury (HPLC\/CV\u2010AFS) were determined for intra\u2010day and inter\u2010day assays, respectively.<\/jats:p>","DOI":"10.1002\/jssc.200500300","type":"journal-article","created":{"date-parts":[[2006,1,17]],"date-time":"2006-01-17T03:47:41Z","timestamp":1137469661000},"page":"248-255","source":"Crossref","is-referenced-by-count":36,"title":["Liquid chromatographic \u2013 cold vapour atomic fluorescence spectrometric determination of mercury species"],"prefix":"10.1002","volume":"29","author":[{"given":"Pavl\u00edna","family":"Houserov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Mat\u011bj\u00ed\u010dek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vlastimil","family":"Kub\u00e1\u0148","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Pavl\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Kom\u00e1rek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-9936(99)00180-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-9936(99)00202-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1023\/A:1018311228405","volume":"97","author":"Wheatley B.","year":"1997","journal-title":"Water Air Soil Poll."},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.17221\/5597-VETMED","volume":"50","author":"Houserov\u00e1 P.","year":"2005","journal-title":"Vet. 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