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Four ODS columns with stationary phases containing different percentages of free silanol groups have been tested. Binary mobile phases of ACN admixed with dichloromethane, tetrahydrofuran, 1\u2010propanol, 2\u2010propanol, ethanol, or acetone have been examined. The choice of modifier depended on the nature of the stationary phase. The more polar solvents were better suited for stationary phases with higher percentage of free silanol groups. Isomeric species were eluted according to chain length, number of double bonds, and the position of the unsaturated acyl chain in the glycerol molecule. Retention increases in the order 20:5 &lt; 22:6 &lt; 18:3 &lt; 20:4 &lt;&lt; 18:2. Within each isomeric pair, the species with unsaturated acyl chain occupying either the <jats:italic>sn<\/jats:italic>\u20101\u2010 or the 3\u2010position were retained preferentially. Complete simultaneous regioselective separation of 10 isomeric triacylglycerols in a single isocratic run on a single ODS column was demonstrated.<\/jats:p>","DOI":"10.1002\/jssc.200500504","type":"journal-article","created":{"date-parts":[[2006,10,25]],"date-time":"2006-10-25T06:44:54Z","timestamp":1161758694000},"page":"2578-2583","source":"Crossref","is-referenced-by-count":67,"title":["Regioselective separation of isomeric triacylglycerols by reversed\u2010phase high\u2010performance liquid chromatography: Stationary phase and mobile phase effects"],"prefix":"10.1002","volume":"29","author":[{"given":"Svetlana","family":"Momchilova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Itabashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boryana","family":"Nikolova\u2010Damyanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnis","family":"Kuksis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531179"},{"key":"e_1_2_1_3_2","unstructured":"Christie W. 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