{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:36:54Z","timestamp":1791153414014,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2006,8,22]],"date-time":"2006-08-22T00:00:00Z","timestamp":1156204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Emodin and physcion are abundant anthraquinone compounds found in the traditional Chinese medicinal herb<jats:italic> Polygonum cuspidatum<\/jats:italic> Sied. et Zucc. In this paper, emodin and physcion were successfully extracted with supercritical CO<jats:sub>2 <\/jats:sub>plus ethanol modifier after the extraction conditions were optimized with uniform design\u2010sequential optimization. Results showed that the ethanol modifier concentration was the main factor for the effective extraction of the emodin. The optimal extraction condition was obtained: 20 MPa, 30\u00b0C, and 95% ethanol, at which the yields of emodin and physcion were 0.616 and 0.178 g\/100 g, respectively. The yield obtained by supercritical fluid extraction (SFE) was a little lower than that obtained by sonication extraction (SE). The crude extract obtained by SFE was further isolated and purified by semipreparative chromatography with the mobile phase composed of methanol\u2010water (90\u00a0:\u00a01, v\/v). Emodin and physcion were obtained with purity 98.6 and 99.1%, respectively, when determined by HPLC, and identification was performed by retention time and UV spectra of the standards. The result suggested that SFE is an alternative and promising method for extraction of the two compounds from <jats:italic>P. cuspidatum<\/jats:italic> owing to its environment\u2010friendly properties and fewer coextracts.<\/jats:p>","DOI":"10.1002\/jssc.200600012","type":"journal-article","created":{"date-parts":[[2006,8,22]],"date-time":"2006-08-22T17:27:44Z","timestamp":1156267664000},"page":"2136-2142","source":"Crossref","is-referenced-by-count":23,"title":["Supercritical CO<sub>2<\/sub> extraction of emodin and physcion from <i>Polygonum cuspidatum <\/i> and subsequent isolation by semipreparative chromatography"],"prefix":"10.1002","volume":"29","author":[{"given":"Hong\u2010Mei","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang\u2010Dong","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Zeng","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Lin","family":"Man","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Editor Committee of Jiangsu New Medical College Encyclopedia of Traditional Chinese Medicine Shanghai Science and Technology Press Shanghai 2001 p. 1329."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)01976-9"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01386-2"},{"key":"e_1_2_1_5_2","first-page":"3890","volume":"55","author":"Zhang L.","year":"1995","journal-title":"Cancer Res."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704205"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(02)00100-3"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/np50083a026"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1746"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2002.tb01332.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.04.026"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2004.03.032"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(95)00045-K"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002849900250"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X96000189"},{"key":"e_1_2_1_16_2","first-page":"230","volume":"29","author":"Goel R. 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