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Here we describe the optimization of an efficient RP\u2010HPLC method for prefractionation of baby hamster kidney cell solubilized proteins. A step gradient elution of acetonitrile was optimized and collected fractions were further examined by SDS\u2010PAGE and 2\u2010DE. By utilizing this method an effective increase in separation power of 2\u2010DE is accomplished. Moreover, we describe the application of this method to expressional proteome analysis of a virally infected cell model.<\/jats:p>","DOI":"10.1002\/jssc.200600140","type":"journal-article","created":{"date-parts":[[2006,10,10]],"date-time":"2006-10-10T10:45:37Z","timestamp":1160477137000},"page":"2284-2291","source":"Crossref","is-referenced-by-count":3,"title":["RP\u2010HPLC prefractionation and its application in expressional proteomics analysis of an <i>in vitro<\/i> viral infection model"],"prefix":"10.1002","volume":"29","author":[{"given":"Behrouz","family":"Vaziri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Rahimpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naser","family":"Eslami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Fayaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heshmat","family":"Rahimian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,10]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401031"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2005.04.010"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.10.030"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000074588"},{"key":"e_1_2_1_6_2","first-page":"479","volume":"261","author":"Barnouin K.","year":"2004","journal-title":"Methods Mol. 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