{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:32:35Z","timestamp":1760887955550,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T00:00:00Z","timestamp":1162252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Among other side effects, administration of anticancer agents is accompanied by manifestations of gastrointestinal toxicity and disturbances of antioxidant balance. The monitoring of these toxic effects in clinical practice is impeded by a dearth of reliable laboratory methods. Therefore, a simple and rapid reversed\u2010phase high\u2010performance liquid chromatography procedure for selective and sensitive determination of retinol, \u03b1\u2010tocopherol, and retinyl esters (retinyl\u2010palmitate and retinyl\u2010stearate) in blood serum has been developed and presented in this study. A Series 200 LC HPLC instrument from Perkin Elmer (Norwalk, USA) with diode\u2010array detector (DAD) was used for the analysis. Separations of retinol, \u03b1\u2010tocopherol, retinyl\u2010palmitate, and retinyl\u2010stearate were performed using a Chromolith Performance RP\u201018e, 100\u00d74.6 mm monolithic column from Merck (Darmstadt, Germany). Gradient elution was used at a flow rate of 3 mL\/min; the mobile phase was methanol\u2010water (95\u00a0:\u00a05, v\/v) for 0\u20132.1 min and methanol\u20102\u2010propanol (60\u00a0:\u00a040, v\/v) for 2.1\u20134.9 min. The total time of analysis was 6 min. The injection volume was 20 \u03bcL and the analysis was performed at ambient temperature. Detection of retinol, \u03b1\u2010tocopherol, and retinyl esters was carried out at 325, 295, and 330 nm, respectively. For practical assessment of the method, the vitamin A absorption test was performed on seven healthy controls as well as on six patients with non\u2010small cell lung carcinoma or head and neck carcinoma previously treated by chemotherapy and\/or radiotherapy, six patients with rectal carcinoma before chemoradiotherapy, four patients with gastrointestinal stromal tumor (GIST) before treatment with imatinib, and a breast cancer patient with chemotherapy\u2010induced diarrhea. Present data demonstrate the feasibility of large scale HPLC determination of vitamin E, vitamin A, and retinyl esters in human serum using a silica monolithic column, and this method may represent a valuable aid in the laboratory monitoring of the toxicity of anticancer therapy.<\/jats:p>","DOI":"10.1002\/jssc.200600153","type":"journal-article","created":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T07:47:19Z","timestamp":1162280839000},"page":"2485-2493","source":"Crossref","is-referenced-by-count":16,"title":["Development and validation of a liquid chromatography method for the simultaneous determination of \u03b1\u2010tocopherol, retinol and retinyl esters in human serum using a monolithic column for the monitoring of anticancer therapy side effects"],"prefix":"10.1002","volume":"29","author":[{"given":"Lubor","family":"Urb\u00e1nek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenka","family":"Kr\u010dmov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagmar","family":"Solichov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bohuslav","family":"Melichar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Opletalov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Solich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,31]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000088957"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.5.1405"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/55.4.857"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/S0022-2275(20)37552-0","volume":"37","author":"v. Reinersdorff D.","year":"1996","journal-title":"J. Lipid Res."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041308"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(03)00008-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.06.064"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.05.139"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2839","DOI":"10.1016\/S0021-9258(18)97256-X","volume":"240","author":"Huang H. S.","year":"1965","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_11_2","unstructured":"European Pharmacopoeia 4th edition (Ph. Eur. 4) Council of Europe Strasbourg 2001 Chromatographic Separation Techniques (2.2.46) 61."},{"key":"e_1_2_1_12_2","unstructured":"International Conference on Harmonization (ICH) Q2A: Text on Validation of Analytical Procedures US FDA Federal Register Vol. 60 March 1995 p. 11260."},{"key":"e_1_2_1_13_2","unstructured":"International Conference on Harmonization (ICH) Q2B: Validation of Analytical Procedures: Methodology US FDA Federal Register Vol. 62 March 1997 p. 27463."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/24.11.1920"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/32.1.35"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0308-8146(93)90015-8"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/clinchem\/40.3.411","volume":"40","author":"Sowell A. L.","year":"1994","journal-title":"Clin. Chem."},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01138-2"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00614-2"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00481-3"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00662-0"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.02.032"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401772"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","unstructured":"Tanaka N. Motokawa M. Kobayashi H. Hosoya K. Ikegami T. in: \u0160vec F. Tennikova T. B. Deyl Z. (Eds.) Monolithic Materials Preparation Properties and Applications Elsevier Amsterdam 2003 pp. 173\u2013196.","DOI":"10.1016\/S0301-4770(03)80024-6"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.10.074"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01570-9"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.12.044"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2006.01.096"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200401827"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.09.045"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2003.10.058"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.02.056"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2004.01.023"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.07.008"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.05.102"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.08.111"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.03.102"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.05.083"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01381-4"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(03)00297-8"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.11.009"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.06.007"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200600153","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.200600153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:58:13Z","timestamp":1760871493000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.200600153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,31]]},"references-count":41,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1002\/jssc.200600153"],"URL":"https:\/\/doi.org\/10.1002\/jssc.200600153","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2006,10,31]]}}}