{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:35:06Z","timestamp":1760920506313,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T00:00:00Z","timestamp":1165968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two cyclolanostane\u2010type saponins, astragalosides I and II, were first identified by TLC\u2010MS\/MS in the ethyl acetate extract of the roots of<jats:italic> Astragalus membranaceus <\/jats:italic>Bge var.<jats:italic> mongholicus <\/jats:italic>(Bge.) Hsiao without chemical reference substances. They were then isolated by high\u2010speed counter\u2010current chromatography with a two\u2010step two\u2010phase solvent system of ethyl acetate\u20102\u2010propanol\u2013water (5:1:5, 50:1:50, v\/v\/v). The quantities of astragalosides I and II isolated from 1 g of the crude extract were 30.2 mg and 16.5 mg, respectively. Their purities were found to be over 95% by HPLC\u2013ELSD analysis. Their chemical structures were confirmed by <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C nuclear magnetic resonance analysis.<\/jats:p>","DOI":"10.1002\/jssc.200600264","type":"journal-article","created":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T07:57:10Z","timestamp":1165996630000},"page":"135-140","source":"Crossref","is-referenced-by-count":12,"title":["Preparative isolation of cyclolanostane\u2010type saponins from <i>Astragalus membranaceus<\/i> Bge. var. <i>mongholicus<\/i> (Bge.) Hsiao by TLC\u2010MS\/MS guided high\u2010speed counter\u2010current chromatography"],"prefix":"10.1002","volume":"30","author":[{"given":"Quan Bin","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing Zheng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Feng","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong Xi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0202279"},{"key":"e_1_2_1_3_2","first-page":"263","volume":"23","author":"Wang Y. 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