{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:01:49Z","timestamp":1780581709876,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2007,7,12]],"date-time":"2007-07-12T00:00:00Z","timestamp":1184198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An HPLC method with diode\u2010array detection, at 355 nm, was developed and validated for the determination of seven tetracyclines (TCs) in milk: minocycline (MNC), TC, oxytetracycline (OTC), methacycline (MTC), demeclocycline (DMC), chlortetracycline (CTC), and doxycycline (DC). Oxalate buffer (pH 4) was used with 20% TCA as a deproteinization agent for the extraction of analytes from milk followed by SPE. The separation was achieved on an Inertsil ODS\u20103, 5 \u03bcm, 250\u00d74 mm<jats:sup>2 <\/jats:sup>analytical column at ambient temperature. The mobile phase, a mixture of A: 0.01 M oxalic acid and B: CH<jats:sub>3<\/jats:sub>CN, was delivered using a gradient program. The procedure was validated according to the European Union decision 2002\/657\/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. Mean recoveries of TCs from spiked milk samples (50, 100, and 200 ng\/g) were 93.8\u2013100.9% for MNC, 96.8\u2013103.7% for OTC, 96.3\u2013101.8% for TC, 99.4\u2013107.2% for DMC, 99.4\u2013102.9% for CTC, 96.3\u2013102.7% for MTC, and 94.6\u2013102.1% for DC. All RSD values were lower than 8.5%. The decision limits CC<jats:sub><jats:italic>a<\/jats:italic><\/jats:sub> calculated by spiking 20 blank milk samples at MRL (100 \u03bcg\/kg) ranged from 101.25 to 105.84 \u03bcg\/kg, while detection capability CC<jats:sub><jats:italic>b<\/jats:italic> <\/jats:sub>from 103.94 to 108.88 \u03bcg\/kg.<\/jats:p>","DOI":"10.1002\/jssc.200700057","type":"journal-article","created":{"date-parts":[[2007,7,12]],"date-time":"2007-07-12T10:03:29Z","timestamp":1184234609000},"page":"2430-2439","source":"Crossref","is-referenced-by-count":45,"title":["Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002\/657\/EC"],"prefix":"10.1002","volume":"30","author":[{"given":"Victoria F.","family":"Samanidou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantina I.","family":"Nikolaidou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis N.","family":"Papadoyannis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,10,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.232-260.2001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.04.013"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.17221\/5681-VETMED","volume":"49","author":"Michalova E.","year":"2004","journal-title":"Vet. Med. Czech."},{"key":"e_1_2_1_5_2","unstructured":"96\/23\/EC Council Directive of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products and repealing Directives 85\/358\/EEC and 86\/469\/EEC and Decisions 89\/187\/EEC and 91\/664\/EEC Off. J. Eur. Commun.1996 L125 10\u201332."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.02.037"},{"key":"e_1_2_1_7_2","unstructured":"2002\/657\/EC Commission Decision of 12 August 2002 implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and the interpretation of results Off. J. Eur. Commun.2002 L221 8\u201336."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2004.07.006"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.04.052"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09298.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)01446-2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(02)00472-1"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002170050171"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1093\/jaoac\/76.2.329","volume":"76","author":"Carson M. C.","year":"1993","journal-title":"J. AOAC Int."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.08.012"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/bms.1200201208"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00171-6"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990915)13:17<1744::AID-RCM709>3.0.CO;2-I"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.08.131"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)87604-4"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00328-X"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01291-0"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500160"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200700057","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.200700057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:26:42Z","timestamp":1760884002000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.200700057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":22,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1002\/jssc.200700057"],"URL":"https:\/\/doi.org\/10.1002\/jssc.200700057","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}