{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T14:22:58Z","timestamp":1784643778610,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2007,9,20]],"date-time":"2007-09-20T00:00:00Z","timestamp":1190246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The separation of nine phenol glycosides \u2013 salicin, salicortin, 2'\u2010acetylsalicortin, populin, tremulacin, salidroside, triandrin, picein and helicin \u2013 by normal phase (NP), reversed phase (RP) HPLC techniques and a coupling of NP and RP monolithic silica columns was studied. Among the above nine compounds only five \u2013 salicin, populin, tremulacin, salidroside and triandrin \u2013 were resolved in an NP system with a mobile phase comprising hexane\/isopropanol\/methanol (87:12:1, v\/v\/v). Optimized separation was performed with two coupled monolithic silica columns of different polarity (bare silica and RP\u201018). The method was applied to verify the presence of salicylic compounds and other phenolic derivatives in the bark of six species from the genus <jats:italic>Salix, <\/jats:italic>namely<jats:italic> S. purpurea, S. daphnoides<\/jats:italic> clone 1095, <jats:italic>S. alba<\/jats:italic> clone 1100,<jats:italic> S. triandra, S. viminalis<\/jats:italic>, and <jats:italic>S. herbacea<\/jats:italic>. Gradient elution with a mobile phase composed of acetonitrile and water containing 0.05% of trifluoroacetic acid, with increasing acetonitrile concentration from 3% to 48%, was chosen as optimal. For the selective detection of the salicylic compounds, an evaporative light scattering detector was employed along with a UV detector. The differences in the composition of phenols in the different plant materials were confirmed. Additionally, it must be emphasized that for the first time the presence of 2\u2032\u2010acetylsalicortin was revealed in<jats:italic> S. alba<\/jats:italic> clone 1100. Furthermore, an SPE\u2010HPLC method was developed for the rapid analysis of the salicin content, analyzed as free and total fraction, in willow barks. The determined concentrations of total salicin varied from 25.4 mg\/g in<jats:italic> S. alba <\/jats:italic>clone 1100 to 96.47 mg\/g in<jats:italic> S. daphnoides<\/jats:italic> clone 1095.<\/jats:p>","DOI":"10.1002\/jssc.200700137","type":"journal-article","created":{"date-parts":[[2007,9,20]],"date-time":"2007-09-20T06:16:33Z","timestamp":1190268993000},"page":"2958-2966","source":"Crossref","is-referenced-by-count":42,"title":["Chromatographic analysis of salicylic compounds in different species of the genus <i>Salix<\/i>"],"prefix":"10.1002","volume":"30","author":[{"given":"Loretta","family":"Pob\u0142ocka\u2010Olech","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Marie","family":"van Nederkassel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvan","family":"Vander Heyden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miros\u0142awa","family":"Krauze\u2010Baranowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Gl\u00f3d","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Baczek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,11,21]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Bisset N. 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Szepesy L. 6thBalaton Symposium on High\u2010Performance Separation Methods \u2013 Book of Abstracts L\u201020 Siofok September 7\u20139 2005."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac980180j"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600103"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.08.057"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014906532428"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-961494"},{"key":"e_1_2_1_24_2","first-page":"434","volume":"6","author":"Poukens\u2010Renwart P.","year":"1993","journal-title":"J. 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