{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T19:04:09Z","timestamp":1782327849300,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T00:00:00Z","timestamp":1193356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A high\u2010performance liquid chromatographic method was developed for the determination of five penicillins: penicillin G (PENG), penicillin V (PENV), oxacillin (OX), cloxacillin (CLO), and dicloxacillin (DICLO), in bovine muscle. Samples were macerated with a mixture of H<jats:sub>2<\/jats:sub>O\/CH<jats:sub>3<\/jats:sub>CN (1:1) and purified using RP\u20108 Adsorbex SPE cartridges after centrifugation, with mean recovery from spiked samples higher than 89%. The separation of the examined penicillins was achieved on an analytical column, an Inertsil C8 5 \u03bcm, 250\u00d74 mm<jats:sup>2<\/jats:sup>, at ambient temperature. The mobile phase consisted of 0.1% TFA\/ACN 50:50 v\/v delivered isocratically at a flow rate of 1.1 mL\/min. Analytes were monitored at 240 nm. The procedure was validated according to the European Union Decision 2002\/657\/EC by means of selectivity, stability, decision limit, detection capability, accuracy, and precision. Method's LOQ values achieved were 54 \u03bcg\/kg for PENG and DICLO, 46 \u03bcg\/kg for PENV, 16 \u03bcg\/kg for OX, and 43 \u03bcg\/kg for DICLO. The detection capabilities (CC<jats:sub>\u03b2<\/jats:sub>) were 73.6 \u03bcg\/kg for PENG, 29.1 \u03bcg\/kg for PENV, 350.6 \u03bcg\/kg for OX, 379.9 \u03bcg\/kg for CLO, and 355.8 \u03bcg\/kg for DICLO. The method was applied to various samples from the local market. Two penicillins were identified by photodiode array (PDA) detection and quantified.<\/jats:p>","DOI":"10.1002\/jssc.200700199","type":"journal-article","created":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T02:43:30Z","timestamp":1193366610000},"page":"3193-3201","source":"Crossref","is-referenced-by-count":30,"title":["Development and validation of an HPLC method for the determination of penicillin antibiotics residues in bovine muscle according to the European Union Decision 2002\/657\/EC"],"prefix":"10.1002","volume":"30","author":[{"given":"Victoria F.","family":"Samanidou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Styliani A.","family":"Nisyriou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis N.","family":"Papadoyannis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,12,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(91)90095-O"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.01.059"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)85678-M"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/10826070701274320"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)00905-6"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00890-0"},{"key":"e_1_2_1_8_2","unstructured":"Council Regulation (EEC) 2377\/90 (OJ L224 p1 18\/08\/90) of 26 June 1990 establishing Community procedures for fixing maximum limits for veterinary drug residues in foodstuffs of animal origin."},{"key":"e_1_2_1_9_2","unstructured":"European Commission Directorate General for Agriculture VIBII2 SANCO\/1805\/2000 Commission Decision laying down analytical methods to be used for detecting certain substances and residues thereof in live animal and animal products according to Council Directive 96\/23\/EC (Revision of Commission Decision 93\/256\/EC)."},{"key":"e_1_2_1_10_2","unstructured":"European Commission 2002\/657\/EC: Commission Decision of 12 August 2002 implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and the interpretation of results Off. 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