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Monolithic columns were checked for their separation behavior for selected dansylated (DNS)\u2010amino acids as well as for cyclophilin 18. Their separation performance was compared to that of a C18\u2010modified silica\u2010based rigid rod (Chromoliths\u00ae). In the separation of dansylated amino acids, retention times were reduced on EB\u2010derived columns, where the peak resolution was significantly better than on a Chromolith\u00ae. This finding was attributed to a larger fraction of small pores (&lt;\u00a02.15 nm) in the EB curing\u2010derived monoliths. 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