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The derivatization was performed at 45\u00b0C for 30 min in borate buffer (pH 8.0). The derivatives were separated on a ZORBAX Eclipse XDB\u2010C8 column (150 mm\u00d74.6 mm id; 5 \u03bcm) and monitored by fluorescence detection (excitation, 469 nm; emission, 512 nm). The LODs (S\/N = 3) for spermine, spermidine, putrescine, cadaverine, and phenethylamine were 0.4, 0.2, 0.3, 0.5, and 0.4 nM, respectively. The developed method has been successfully applied to the determination of biogenic amines in human plasma of three healthy volunteers and four cancer patients. Average recoveries for plasma samples ranged from 94 to 106% and coefficients of variation ranged from 1.8 to 4.6%. Deproteinization of plasma was accomplished with ACN to precipitate interfering substances and the centrifuged supernatant was used directly for analysis.<\/jats:p>","DOI":"10.1002\/jssc.200700399","type":"journal-article","created":{"date-parts":[[2008,3,11]],"date-time":"2008-03-11T08:16:49Z","timestamp":1205223409000},"page":"990-998","source":"Crossref","is-referenced-by-count":18,"title":["Quantification of biogenic amines in human plasma based on the derivatization with <i>N<\/i>\u2010hydroxy\u2010succinimidyl fluorescein\u2010<i>O<\/i>\u2010acetate by high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"31","author":[{"given":"Ying\u2010Hua","family":"Deng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua\u2010Shan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Lan","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac030355b"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(95)00032-1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2006.08.028"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1987.tb11318.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19800101)45:1<108::AID-CNCR2820450120>3.0.CO;2-W"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2007.02.003"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00395-4"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00669-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1093\/clinchem\/21.7.860","volume":"21","author":"Russell D. 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