{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:27:16Z","timestamp":1760920036240,"version":"build-2065373602"},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,12,13]],"date-time":"2007-12-13T00:00:00Z","timestamp":1197504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fluorescence detectors are about three orders of magnitude more sensitive and specific making them ideal for trace analysis and complex sample matrices. However, temperature dependence of the signal is a disadvantage to fluorescence detectors. We have previously reported degradation of malondialdehyde (MDA)\u2013thiobarbituric acid (TBA) adduct at room temperature. The present experiments tested the notion that the detector response may be blunted due to increase in temperature over time. Repeated injections of the same standard curve over 4 h found a significant effect of time on the slopes of peak area\u2013concentration curves. When the samples were iced and injected alternating with ambient temperature standard curve samples, no differences in slopes were seen between iced and ambient temperature samples. Cooling the housing of the fluorescence lamp significantly increased the fluorescence in the samples. Fluorescence increased 2.5% (95% confidence interval, 1.5\u20133.6%) for each 1\u00b0C fall in temperature. MDA\u2013TBA adduct remained stable at room temperature. Since the fluorescence signal is temperature sensitive, letting the detector warm up for 2 h to obtain a steady temperature is more likely to give reproducible results compared to a detector that has not warmed up. These results have implications for other applications utilizing fluorescence detectors.<\/jats:p>","DOI":"10.1002\/jssc.200700415","type":"journal-article","created":{"date-parts":[[2007,12,13]],"date-time":"2007-12-13T09:49:10Z","timestamp":1197539350000},"page":"128-132","source":"Crossref","is-referenced-by-count":6,"title":["Temperature sensitivity and fluorescence detection"],"prefix":"10.1002","volume":"31","author":[{"given":"Rajiv","family":"Agarwal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,10]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","unstructured":"Snyder L. R. Kirkland J. J. Glajch J. L. Practical HPLC Method Development Wiley New York 1997.","DOI":"10.1002\/9781118592014"},{"key":"e_1_2_1_3_2","unstructured":"Guilbault G. G. Practical Fluorescence M. Dekker New York 1990."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00273-8"},{"key":"e_1_2_1_5_2","unstructured":"Glantz S. A. Slinker B. K. Primer of Applied Regression & Analysis of Variance McGraw\u2010Hill New York 2001."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)80078-I"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200700415","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.200700415","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.200700415","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:23:29Z","timestamp":1760869409000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.200700415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1002\/jssc.200700415"],"URL":"https:\/\/doi.org\/10.1002\/jssc.200700415","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}